Cellular Imaging Core
The Cellular Imaging Core (CIC) is an optical microscopy core facility with a range of digital imaging microscopy systems for studying cells and tissues. CIC offers a range of wide-field and confocal microscope imaging systems. In addition to equipment, we also offer help in choosing the appropriate fluorescent indicators and constructs, as well as assistance in image processing and analysis.
|IonOptix Calcium Imaging||B15, EBRC Building, 650 Albany|
|Olympus DSU Wide-field and Spinning Disk Confocal||B15, EBRC Building, 650 Albany|
|Leica SP5 Confocal Microscope||B15, EBRC Building, 650 Albany|
|Nikon Deconvolution Wide-Field Epifluorescence System||B15, EBRC Building, 650 Albany|
|Zeiss LSM 710-Live Duo Confocal with 2-Photon Capability||B15, EBRC Building, 650 Albany|
|Nexcelom Celigo, Image-based Microplate Reader||B15, EBRC Building, 650 Albany|
|Olympus Stereo Fluorescence Imaging Microscope||B15, EBRC Building, 650 Albany|
|Olympus Upright Wide-field, spinning Disk, and IHC Imaging||B15, EBRC Building, 650 Albany|
|Two-Photon Confocal||B15, EBRC Building, 650 Albany|
Data analysis and storage:
The Core also offers state of the art image processing and analysis capabilities including 5 custom built workstations housed in the core space and a 300 Terabyte Isilon high-availability server. The Core also maintains several copies of Molecular Devices Meta Imaging Series software, Nikon NIS Elements, Zeiss Zen, and Leica AF for offline use as well as offering assistance with using NIH ImageJ. Use of the image analysis facility and software is provided free of charge.
- The Core is open 24 hr/day, 7 days/wk for trained users working independently. Training is available weekdays from 11:30 am to 8 pm by appointment (several days advanced notice is requested).
- Each lab can book a maximum of 10 hours of day time use or 15 hours of total use per week on each instrument.
- This rule does not apply for “Next Day” reservation (booking of time for the coming day).
- Therefore, frequent users are welcome to fill in the open gaps but they can book these hours only 1 day before the use.
We would greatly appreciate it if authors would acknowledge the Cellular Imaging Core in their publications containing data obtained with the equipment and/or assistance of Core personnel.
Orian Shirihai, MD, Ph.D.
Michael T. Kirber, Ph.D.
Office: (617) 638-7153