Zeiss LSM 710 NLO Microscope System

The Zeiss LSM 710 NLO microscope system equipped with 6 visible laser lines (458nm, 488nm, 514nm, 561nm, 594nm, 633nm), a 405nm solid state laser as well as a tunable femtosecond IR pulse laser for multiphoton excitation (Coherent Chameleon Vision–S).

In addition to two descanned internal detectors and a spectral detector which enables lambda scanning and linear unmixing of fluorescent probes, the system has two highly sensitive gallium arsenide non-descanned detectors for collection of emitted light from two-photon experiments. The laser scanning system is coupled to an upright microscope with a motorized stage and is complete with a variety of objective lenses (10X, 20X, 40X, 63X).

Applications of the system include:

Optical sectioning of fluorescent tissue using confocal or multiphoton imaging
Time-lapse imaging of living tissue or cell culture
Tile scanning and stitching of composite images
3D reconstruction of biological tissue/cells
In vivo time-lapse imaging
FRAP, FRET, Bleaching

BU Users Rates	External Rates
$38/hour unassisted $75/hour assisted $75/hour training	$75/hour unassisted $129/hour assisted $129/hour training

How to Schedule

Please login to iLab system to schedule equipment time or services. For new users please follow the steps outlined in Information for New Users.

Contact

Vickery Trinkaus-Randall, PhD
Core Director
(617) 358-1235 |vickery@bu.edu

Location

BUSM Housman Medical Research Center
72 E. Concord Street, R Building R-815
Boston, MA 02118

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