Our laboratory is interested in the wound response and repair of epithelium, neurons and extracellular matrix. 1. In the healthy cornea there are no blood vessels and thus the tissue provides an excellent model to study the communication between nerves and epithelium following injury. Primary neurons are co-cultured with epithelial cells to ask how signaling pathways communicate. These include but are not limited to glutamatergic and purinergic receptor-mediated calcium transients. 2. In addition to these signaling events we have found that when epithelial cells are injured nucleotides are released immediately and cause a distinct phosphorylation of EGF receptor residues that ultimately affect the ability of a cell to migrate. 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. Our experiments are conducted using a number of organ culture and cell models and use biochemical, cell and molecular techniques. 4. Last but not least we are interested in the role of long sugar chains or glycosaminoglycans on the formation of oligomers and fibrils in light chain amyloid disease using atomic force microscopy, negative staining and biochemical technologies.
- Professor, Ophthalmology, Boston University School of Medicine
- Co-Director, MD/PhD Program, Boston University School of Medicine, Graduate Medical Sciences
- Member, Amyloidosis Center, Boston University
- Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Graduate Medical Sciences
- Member, Genome Science Institute, Boston University
- University of Wisconsin-Madison, PhD
- Kenyon College, BA
- Published on 11/7/2020
Lee YK, Segars KL, Trinkaus-Randall V. Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells. Methods Mol Biol. 2020 Nov 07. PMID: 33159251.
- Published on 2/7/2020
Xu P, Londregan A, Rich C, Trinkaus-Randall V. Changes in Epithelial and Stromal Corneal Stiffness Occur with Age and Obesity. Bioengineering (Basel). 2020 Feb 07; 7(1). PMID: 32046198.
- Published on 1/13/2020
Stepp MA, Trinkaus-Randall V. In memoriam: James D. Zieske, Ph.D. (1954-2020). Exp Eye Res. 2020 Feb; 191:107934. PMID: 32063264.
- Published on 1/13/2020
Stepp MA, Trinkaus-Randall V. In memoriam: James D. Zieske, Ph.D. (1954-2020). Exp Eye Res. 2020 Feb; 191:107934. PMID: 31945322.
- Published on 4/24/2019
Lee Y, Kim MT, Rhodes G, Sack K, Son SJ, Rich CB, Kolachalama VB, Gabel CV, Trinkaus-Randall V. Sustained Ca2+ mobilizations: A quantitative approach to predict their importance in cell-cell communication and wound healing. PLoS One. 2019; 14(4):e0213422. PMID: 31017899.
- Published on 4/5/2019
Onochie OE, Onyejose AJ, Rich CB, Trinkaus-Randall V. The Role of Hypoxia in Corneal Extracellular Matrix Deposition and Cell Motility. Anat Rec (Hoboken). 2020 06; 303(6):1703-1716. PMID: 30861330.
- Published on 1/14/2019
Onochie OE, Zollinger A, Rich CB, Smith M, Trinkaus-Randall V. Epithelial cells exert differential traction stress in response to substrate stiffness. Exp Eye Res. 2019 04; 181:25-37. PMID: 30653966.
- Published on 6/5/2018
Kneer K, Green MB, Meyer J, Rich CB, Minns MS, Trinkaus-Randall V. High fat diet induces pre-type 2 diabetes with regional changes in corneal sensory nerves and altered P2X7 expression and localization. Exp Eye Res. 2018 10; 175:44-55. PMID: 29883639.
- Published on 2/27/2018
Lee A, Karamichos D, Onochie OE, Hutcheon AEK, Rich CB, Zieske JD, Trinkaus-Randall V. Hypoxia modulates the development of a corneal stromal matrix model. Exp Eye Res. 2018 05; 170:127-137. PMID: 29496505.
- Published on 5/31/2017
Barrios J, Patel KR, Aven L, Achey R, Minns MS, Lee Y, Trinkaus-Randall VE, Ai X. Early life allergen-induced mucus overproduction requires augmented neural stimulation of pulmonary neuroendocrine cell secretion. FASEB J. 2017 Sep; 31(9):4117-4128. PMID: 28566470.
View 104 more publications: View full profile at BUMC