Our laboratory is interested in the response to injury. Injury may be a traumatic event or a change in the integrity of the tissue that occurs from an onset of disease. Our lab uses the cornea to study cell repair as it is a simple model tissue as it is avascular yet highly innervated tissue. In addition, corneal disease and injury remain a major cause of blindness (World Health Organization) and affects over 10 million people worldwide. Diseases such as Type II Diabetes present an overwhelming problem to the cornea with rapid changes in innervation and wound repair. Our lab uses a number of in vitro, organ culture and in vivo models to examine wound repair using a pre-Type II diabetic model. Recently we have shown that changes in the sensory innervation and expression profile in the cornea can occur rapidly and may alter cell communication that underlies collective migration. As the cornea is avascular the wound response is sensitive to soluble factors released with injury that induce downstream signaling events. We speculate that the communication occurs through a family of proteins called pannexins. In addition, the proper repair of the pre-diabetic tissue may be compromised by a family of proteins along the surface of epithelial cells where the polarity of the proteins is misregulated. To determine cell communication after injury we perform high resolution live imaging technologies to examine changes in calcium mobilization, cytoskeletal reorganization and cell motility over time. These are combined with confocal imaging of fluorescent probes in fixed tissues. These are analyzed using a number of MATLAB scripts. These studies require an understanding of the dynamics between matrix molecules and growth factors and how they are released from various depots.
Kristen Segars – MD, PhD student
Anwuli Onyejose – MAMS student
Molly Kalker – MAMS student
Kyubi Yang – undergraduate student
- Kneer K, Green MB, Meyer J, Rich CB, Minns MS, Trinkaus-Randall V. High fat diet induces pre-type 2 diabetes with regional changes in corneal sensory nerves and altered P2X7 expression and localization. Exp Eye Res. 2018 Jun 05; 175:44-55.. PMID: 29883639
- Lee A, Karamichos D, Onochie OE, Hutcheon AEK, Rich CB, Zieske JD, Trinkaus-Randall V. Hypoxia modulates the development of a corneal stromal matrix model. Exp Eye Res. 2018 May; 170:127-137. PMID: 29496505.
- Barrios J, Patel KR, Aven L, Achey R, Minns MS, Lee Y, Trinkaus-Randall VE, Ai X. Early life allergen-induced mucus overproduction requires augmented neural stimulation of pulmonary neuroendocrine cell secretion. FASEB J. 2017 May 31. PMID: 28566470.
- Minns MS, Trinkaus-Randall V. Purinergic Signaling in Corneal Wound Healing: A Tale of 2 Receptors. J Ocul Pharmacol Ther. 2016 Oct; 32(8):498-503. PMID: 27643999. View in: PubMed
- Minns MS, Teicher G, Rich CB, Trinkaus-Randall V. Purinoreceptor P2X7 Regulation of Ca(2+) Mobilization and Cytoskeletal Rearrangement Is Required for Corneal Reepithelialization after Injury. Am J Pathol. 2016 Feb; 186(2):285-96. PMID: 26683661. View in: PubMed
- Derricks KE, Trinkaus-Randall V, Nugent MA. Extracellular matrix stiffness modulates VEGF calcium signaling in endothelial cells: individual cell and population analysis. Integr Biol (Camb). 2015 Sep; 7(9):1011-25. PMID: 26183123. View in: PubMed
- Sanderson J, Dartt DA, Trinkaus-Randall V, Pintor J, Civan MM, Delamere NA, Fletcher EL, Salt TE, Grosche A, Mitchell CH. Purines in the eye: recent evidence for the physiological and pathological role of purines in the RPE, retinal neurons, astrocytes, Müller cells, lens, trabecular meshwork, cornea and lacrimal gland. Exp Eye Res. 2014 Oct; 127:270-9. PMID: 25151301. View in: PubMed
- Karamichos D, Hutcheon AE, Rich CB, Trinkaus-Randall V, Asara JM, Zieske JD. In vitro model suggests oxidative stress involved in keratoconus disease. Sci Rep. 2014; 4:4608. PMID: 24714342. View in: PubMed
- Lee A, Derricks K, Minns M, Ji S, Chi C, Nugent MA, Trinkaus-Randall V. Hypoxia-induced changes in Ca(2+) mobilization and protein phosphorylation implicated in impaired wound healing. Am J Physiol Cell Physiol. 2014 May 15; 306(10):C972-85. PMID: 24671101. View in: PubMed
- Stepp MA, Zieske JD, Trinkaus-Randall V, Kyne BM, Pal-Ghosh S, Tadvalkar G, Pajoohesh-Ganji A. Wounding the cornea to learn how it heals. Exp Eye Res. 2014 Apr; 121:178-93. PMID: 24607489. View in: PubMed
- Karamichos D, Rich CB, Zareian R, Hutcheon AE, Ruberti JW, Trinkaus-Randall V, Zieske JD. TGF-ß3 stimulates stromal matrix assembly by human corneal keratocyte-like cells. Invest Ophthalmol Vis Sci. 2013 Oct; 54(10):6612-9. PMID: 24022012. View in: PubMed
- Kehasse A, Rich CB, Lee A, McComb ME, Costello CE, Trinkaus-Randall V. Epithelial wounds induce differential phosphorylation changes in response to purinergic and EGF receptor activation. Am J Pathol. 2013 Dec; 183(6):1841-52. PMID: 24095926. View in: PubMed
- Chi C, Trinkaus-Randall V. New insights in wound response and repair of epithelium. J Cell Physiol. 2013 May; 228(5):925-9. PMID: 23129239. View in: PubMed
Complete list can be found at BU Profiles