Vickery E. Trinkaus-Randall, PhD
Professor, Biochemistry & Cell Biology
Biography
Our laboratory is interested in the wound response and repair of epithelium, neurons and extracellular matrix. 1. In the healthy cornea there are no blood vessels and thus the tissue provides an excellent model to study the communication between nerves and epithelium following injury. Primary neurons are co-cultured with epithelial cells to ask how signaling pathways communicate. These include but are not limited to glutamatergic and purinergic receptor-mediated calcium transients. 2. In addition to these signaling events we have found that when epithelial cells are injured nucleotides are released immediately and cause a distinct phosphorylation of EGF receptor residues that ultimately affect the ability of a cell to migrate. 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. Our experiments are conducted using a number of organ culture and cell models and use biochemical, cell and molecular techniques. 4. Last but not least we are interested in the role of long sugar chains or glycosaminoglycans on the formation of oligomers and fibrils in light chain amyloid disease using atomic force microscopy, negative staining and biochemical technologies.
Other Positions
- Professor, Ophthalmology, Boston University Chobanian & Avedisian School of Medicine
- Member, Amyloidosis Center, Boston University
- Member, Genome Science Institute, Boston University
- Co-Director, MD/PhD Program, Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences
- Graduate Faculty (Primary Mentor of Grad Students), Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences
Education
- University of Wisconsin-Madison, PhD
- Kenyon College, BA
Publications
- Published on 8/12/2024
Segars KL, Azzari N, Cole M, Kushimi L, Rapaka S, Rich CB, Trinkaus-Randall V. Diverse calcium signaling profiles regulate migratory behavior in avascular wound healing and aberrant signal hierarchy occurs early in diabetes. Am J Physiol Cell Physiol. 2024 Oct 01; 327(4):C1051-C1072. PMID: 39129489.
Read at: PubMed - Published on 12/21/2023
Azzari NA, Segars KL, Rapaka S, Kushimi L, Rich CB, Trinkaus-Randall V. Aberrations in Cell Signaling Quantified in Diabetic Murine Globes after Injury. Cells. 2023 Dec 21; 13(1). PMID: 38201230.
Read at: PubMed - Published on 8/30/2023
Segars KL, Trinkaus-Randall V. Glycosaminoglycans: Roles in wound healing, formation of corneal constructs and synthetic corneas. Ocul Surf. 2023 Oct; 30:85-91. PMID: 37657650.
Read at: PubMed - Published on 10/6/2022
Segars KL, Azzari NA, Gomez S, Rich CB, Trinkaus-Randall V. Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder. J Vis Exp. 2022 Oct 06; (188). PMID: 36282717.
Read at: PubMed - Published on 5/5/2022
Segars KL, Azzari NA, Gomez S, Machen C, Rich CB, Trinkaus-Randall V. Age Dependent Changes in Corneal Epithelial Cell Signaling. Front Cell Dev Biol. 2022; 10:886721. PMID: 35602595.
Read at: PubMed - Published on 7/13/2021
Rhodes G, Segars KL, Lee YK, Hutcheon AEK, Rich CB, Trinkaus-Randall V. Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium. Anal Cell Pathol (Amst). 2021; 2021:4793338. PMID: 34336553.
Read at: PubMed - Published on 1/1/2021
Lee YK, Segars KL, Trinkaus-Randall V. Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells. Methods Mol Biol. 2021; 2346:11-20. PMID: 33159251.
Read at: PubMed - Published on 2/7/2020
Xu P, Londregan A, Rich C, Trinkaus-Randall V. Changes in Epithelial and Stromal Corneal Stiffness Occur with Age and Obesity. Bioengineering (Basel). 2020 Feb 07; 7(1). PMID: 32046198.
Read at: PubMed - Published on 1/13/2020
Stepp MA, Trinkaus-Randall V. In memoriam: James D. Zieske, Ph.D. (1954-2020). Exp Eye Res. 2020 Feb; 191:107934. PMID: 32063264.
Read at: PubMed - Published on 1/13/2020
Stepp MA, Trinkaus-Randall V. In memoriam: James D. Zieske, Ph.D. (1954-2020). Exp Eye Res. 2020 Feb; 191:107934. PMID: 31945322.
Read at: PubMed
View 110 more publications: View full profile at BUMC