Membrane and Receptor Biology:
Our interests center around the structure and function of cell membranes, receptor-ligand interactions and transmembrane signaling mechanisms. A combination of biochemical, chemical and biophysical approaches are used to probe: (1) membrane lipid conformation, structure, properties and interactions, (2) membrane receptor-lipid interactions and (3) membrane receptor-ligand interactions. Current approaches involve the isolation of membrane-associated proteins and lipids (e.g. low density lipoprotein (LDL) receptor, insulin receptor, integrins, gangliosides), their reconstitution with well characterized lipid monolayer and bilayer matrices and structural studies of the protein-lipid assemblies. Ligand binding studes include LDL receptor/LDL, insulin receptor/insulin, and ganglioside/toxin interactions. The biochemical methods involved include lipid and protein isolation, gel/affinity chromatography, antibody techniques, immunoblotting, detergent solubilization, lipid-protein reconstitution, etc. Chemical methods include lipid sythesis, TLC, HPLC, IR and NMR. The biophysical approach involves x-ray diffraction, protein crystallography, electron microscopy/image reconstruction, surface chemistry and calorimetric and spectroscopic (CD, NMR) methods.
- Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Division of Graduate Medical Sciences
- Professor, Biochemistry, Boston University School of Medicine
- University of Nottingham, PhD
- University of Nottingham, BSc
- Published on 8/24/2009
Doyle DA, Shipley GG. Membranes: editorial overview. Curr Opin Struct Biol. 2009 Aug; 19(4):369-71. PMID: 19709877.
- Published on 9/29/2000
Jeon H, Shipley GG. Localization of the N-terminal domain of the low density lipoprotein receptor. J Biol Chem. 2000 Sep 29; 275(39):30465-70. PMID: 10889195.
- Published on 9/29/2000
Jeon H, Shipley GG. Vesicle-reconstituted low density lipoprotein receptor. Visualization by cryoelectron microscopy. J Biol Chem. 2000 Sep 29; 275(39):30458-64. PMID: 10889196.
- Published on 8/1/2000
Shipley GG. Bilayers and nonbilayers: structure, forces and protein crystallization. Curr Opin Struct Biol. 2000 Aug; 10(4):471-3. PMID: 10981637.
- Published on 7/1/2000
Saxena K, Duclos RI, Sripada PK, Shipley GG. Unusual hydration properties of C16:0 sulfatide bilayer membranes. Biophys J. 2000 Jul; 79(1):385-93. PMID: 10866964.
- Published on 1/1/2000
Saxena K, Zimmermann P, Schmidt RR, Shipley GG. Bilayer properties of totally synthetic C16:0-lactosyl-ceramide. Biophys J. 2000 Jan; 78(1):306-12. PMID: 10620294.
- Published on 12/3/1999
Woldin CN, Hing FS, Lee J, Pilch PF, Shipley GG. Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor. J Biol Chem. 1999 Dec 3; 274(49):34981-92. PMID: 10574975.
- Published on 5/1/1999
Saxena K, Duclos RI, Zimmermann P, Schmidt RR, Shipley GG. Structure and properties of totally synthetic galacto- and gluco-cerebrosides. J Lipid Res. 1999 May; 40(5):839-49. PMID: 10224153.
- Published on 12/16/1997
Saxena K, Shipley GG. Structural studies of detergent-solubilized and vesicle-reconstituted low-density lipoprotein (LDL) receptor. Biochemistry. 1997 Dec 16; 36(50):15940-8. PMID: 9398328.
- Published on 6/18/1996
Maulik PR, Shipley GG. N-palmitoyl sphingomyelin bilayers: structure and interactions with cholesterol and dipalmitoylphosphatidylcholine. Biochemistry. 1996 Jun 18; 35(24):8025-34. PMID: 8672507.
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