C. James McKnight, PhD

Associate Professor, Physiology & Biophysics

C. McKnight
617.358.8476
700 Albany St Ctr for Adv Biomed Res

Biography

My expertise is in protein structure, function, dynamics and folding and chemistry. Methods used include molecular biology, chromatography, NMR spectroscopy, X-ray crystallography, UV/Vis and circular dichroism spectroscopy, electron microscopy, molecular modeling and dynamics. We look at actin binding proteins (especially those containing villin-type headpiece domains) and lipid binding proteins including apolipoprotein B, the essential protein component of low density lipoprotein (LDL, the bad cholesterol) whose elevated levels are correlated with atherosclerosis, stroke, heart and cardiovascular disease.

Other Positions

  • Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Graduate Medical Sciences

Education

  • University of Texas Southwestern Medical, PhD
  • University of Delaware, MA
  • Washington College, BS

Classes Taught

  • MED MS 146 M1
  • BY763
  • GMS FC713
  • GMSFC701
  • GMSFC703
  • GMSFC711
  • GMSOH730
  • GMSPH731
  • MS 145
  • MS 145
  • MS 145 M3
  • SDM MD514

Publications

  • Published on 5/26/2020

    Nichols PJ, Falconer I, Griffin A, Mant C, Hodges R, McKnight CJ, Vögeli B, Vugmeyster L. Deuteration of nonexchangeable protons on proteins affects their thermal stability, side-chain dynamics, and hydrophobicity. Protein Sci. 2020 07; 29(7):1641-1654. PMID: 32356390.

    Read at: PubMed
  • Published on 8/18/2018

    Vugmeyster L, Griffin A, Ostrovsky D, Bhattacharya S, Nichols PJ, McKnight CJ, Vögeli B. Correlated motions of C'-N and Ca-Cß pairs in protonated and per-deuterated GB3. J Biomol NMR. 2018 Oct; 72(1-2):39-54. PMID: 30121872.

    Read at: PubMed
  • Published on 2/21/2018

    Miears HL, Gruber DR, Horvath NM, Antos JM, Young J, Sigurjonsson JP, Klem ML, Rosenkranz EA, Okon M, McKnight CJ, Vugmeyster L, Smirnov SL. Plant Villin Headpiece Domain Demonstrates a Novel Surface Charge Pattern and High Affinity for F-Actin. Biochemistry. 2018 03 20; 57(11):1690-1701. PMID: 29444403.

    Read at: PubMed
  • Published on 9/27/2017

    Xiao R, Dane EL, Zeng J, McKnight CJ, Grinstaff MW. Synthesis of Altrose Poly-amido-saccharides with ß-N-(1?2)-d-amide Linkages: A Right-Handed Helical Conformation Engineered in at the Monomer Level. J Am Chem Soc. 2017 10 11; 139(40):14217-14223. PMID: 28902504.

    Read at: PubMed
  • Published on 9/13/2017

    Falconer IB, Mant CT, McKnight CJ, Vugmeyster L, Hodges R. Optimized purification of a fusion protein by reversed-phase high performance liquid chromatography informed by the linear solvent strength model. J Chromatogr A. 2017 Oct 27; 1521:44-52. PMID: 28942999.

    Read at: PubMed
  • Published on 5/12/2016

    Chin SL, Lu Q, Dane EL, Dominguez L, McKnight CJ, Straub JE, Grinstaff MW. Combined Molecular Dynamics Simulations and Experimental Studies of the Structure and Dynamics of Poly-Amido-Saccharides. J Am Chem Soc. 2016 05 25; 138(20):6532-40. PMID: 27119983.

    Read at: PubMed
  • Published on 10/29/2015

    Harpole KW, O'Brien ES, Clark MA, McKnight CJ, Vugmeyster L, Wand AJ. The unusual internal motion of the villin headpiece subdomain. Protein Sci. 2016 Feb; 25(2):423-32. PMID: 26473993.

    Read at: PubMed
  • Published on 8/12/2015

    Raina D, Agarwal P, Lee J, Bharti A, McKnight CJ, Sharma P, Kharbanda S, Kufe D. Characterization of the MUC1-C Cytoplasmic Domain as a Cancer Target. PLoS One. 2015; 10(8):e0135156. PMID: 26267657.

    Read at: PubMed
  • Published on 7/17/2015

    Chen L, Brown JW, Mok YF, Hatters DM, McKnight CJ. The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). J Biol Chem. 2015 Jul 17; 290(29):17808. PMID: 26188045.

    Read at: PubMed
  • Published on 6/11/2015

    Brown JW, Bullitt E, Sriswasdi S, Harper S, Speicher DW, McKnight CJ. The Physiological Molecular Shape of Spectrin: A Compact Supercoil Resembling a Chinese Finger Trap. PLoS Comput Biol. 2015 Jun; 11(6):e1004302. PMID: 26067675.

    Read at: PubMed

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