C. James McKnight, PhD

Associate Professor, Physiology & Biophysics

C. McKnight
(617) 638-4042
700 Albany St Ctr for Adv Biomed Res

Biography

My expertise is in protein structure, function, dynamics and folding and chemistry. Methods used include molecular biology, chromatography, NMR spectroscopy, X-ray crystallography, UV/Vis and circular dichroism spectroscopy, electron microscopy, molecular modeling and dynamics. We look at actin binding proteins (especially those containing villin-type headpiece domains) and lipid binding proteins including apolipoprotein B, the essential protein component of low density lipoprotein (LDL, the bad cholesterol) whose elevated levels are correlated with atherosclerosis, stroke, heart and cardiovascular disease.

Other Positions

  • Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Division of Graduate Medical Sciences

Education

  • University of Texas Southwestern Medical, PhD
  • University of Delaware, MA
  • Washington College, BS

Publications

  • Published on 9/27/2017

    Xiao R, Dane EL, Zeng J, McKnight CJ, Grinstaff MW. Synthesis of Altrose Poly-amido-saccharides with ß-N-(1?2)-d-amide Linkages: A Right-Handed Helical Conformation Engineered in at the Monomer Level. J Am Chem Soc. 2017 Oct 11; 139(40):14217-14223. PMID: 28902504.

    Read at: PubMed
  • Published on 9/13/2017

    Falconer IB, Mant CT, McKnight CJ, Vugmeyster L, Hodges R. Optimized purification of a fusion protein by reversed-phase high performance liquid chromatography informed by the linear solvent strength model. J Chromatogr A. 2017 Oct 27; 1521:44-52. PMID: 28942999.

    Read at: PubMed
  • Published on 5/12/2016

    Chin SL, Lu Q, Dane EL, Dominguez L, McKnight CJ, Straub JE, Grinstaff MW. Combined Molecular Dynamics Simulations and Experimental Studies of the Structure and Dynamics of Poly-Amido-Saccharides. J Am Chem Soc. 2016 May 25; 138(20):6532-40. PMID: 27119983.

    Read at: PubMed
  • Published on 10/29/2015

    Harpole KW, O'Brien ES, Clark MA, McKnight CJ, Vugmeyster L, Wand AJ. The unusual internal motion of the villin headpiece subdomain. Protein Sci. 2016 Feb; 25(2):423-32. PMID: 26473993.

    Read at: PubMed
  • Published on 8/12/2015

    Raina D, Agarwal P, Lee J, Bharti A, McKnight CJ, Sharma P, Kharbanda S, Kufe D. Characterization of the MUC1-C Cytoplasmic Domain as a Cancer Target. PLoS One. 2015; 10(8):e0135156. PMID: 26267657.

    Read at: PubMed
  • Published on 7/17/2015

    Chen L, Brown JW, Mok YF, Hatters DM, McKnight CJ. The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). J Biol Chem. 2015 Jul 17; 290(29):17808. PMID: 26188045.

    Read at: PubMed
  • Published on 6/11/2015

    Brown JW, Bullitt E, Sriswasdi S, Harper S, Speicher DW, McKnight CJ. The Physiological Molecular Shape of Spectrin: A Compact Supercoil Resembling a Chinese Finger Trap. PLoS Comput Biol. 2015 Jun; 11(6):e1004302. PMID: 26067675.

    Read at: PubMed
  • Published on 12/1/2014

    Jiang ZG, Wu Y, Csizmadia E, Feldbrügge L, Enjyoji K, Tigges J, Toxavidis V, Stephan H, Müller CE, McKnight CJ, Moss A, Robson SC. Erratum to: Characterization of circulating microparticle-associated CD39 family ecto-nucleotidases in human plasma. Purinergic Signal. 2014 Dec; 10(4):855. PMID: 25301668.

    Read at: PubMed
  • Published on 8/28/2014

    Jiang ZG, Wu Y, Csizmadia E, Feldbrügge L, Enjyoji K, Tigges J, Toxavidis V, Stephan H, Müller CE, Müller CE, McKnight CJ, Moss A, Robson SC. Characterization of circulating microparticle-associated CD39 family ecto-nucleotidases in human plasma. Purinergic Signal. 2014 Dec; 10(4):611-8. PMID: 25165006.

    Read at: PubMed
  • Published on 8/6/2014

    Miller SA, Burnett JR, Leonis MA, McKnight CJ, van Bockxmeer FM, Hooper AJ. Novel missense MTTP gene mutations causing abetalipoproteinemia. Biochim Biophys Acta. 2014 Oct; 1842(10):1548-54. PMID: 25108285.

    Read at: PubMed

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