The Mostoslavsky Lab
M.D., National University of Tucuman, Argentina
Ph.D., The Hebrew University, Jerusalem, Israel Post Doc Harvard Medical School
General field of research:
Stem Cells; iPS cells; HSCs; ISCs; Reprogramming; Self-renewal; Cell therapy; Gene transfer
Affiliations other than medicine:
Evans Center for Interdisciplinary Biomedical Research
Department of Microbiology
650 Albany Street, Suite 513
Phone: (617)-638 6532
650 Albany Street, Suite 520
Phone: (617)-638 8332
Other research websites:
iPS cells; Reprogramming; Lentiviral stem cell cassette; Intestinal differentiation
Summary of research interest:
The Mostoslavsky Lab is a basic science laboratory in the Section of Gastroenterology in the Department of Medicine at Boston University. Our goal is to advance our understanding of stem cell biology with a focus on their genetic manipulation via gene transfer and their potential use for stem cell-based therapy. We believe that by discovering the mechanisms involved in stem cell self-renewal and differentiation we will be able to manipulate stem cell fate and use it as the basis for the correction of several diseases. Project areas in the lab focuses on the use of different stem cell populations, including embryonic stem cells, induced Pluripotent Stem (iPS) cells, hematopoietic stem cells and intestinal stem cells and their genetic manipulation by lentiviral vectors.
Sommer, C., Stadtfeld, M., Murphy, G.J., Hochedlinger, K., Kotton, D.N., and Mostoslavsky, G. 2008. iPS Cell Generation Using a Single Lentiviral Stem Cell Cassette. Stem Cells.
Pan, H., Mostoslavsky, G., Eruslanov, E., Kotton, D.N., and Kramnik, I. 2008. Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages. J. Immunol. Methods. Jan 1;329(1-2):31-44.
De Coppi, P., Bartsch, G. Jr., Siddiqui M.M., Xu, T., Santos, C.C., Perin, L., Mostoslavsky, G., Serre, A.C., Snyder, E.Y., Yoo, J.J., Furth, M.E., Soker, S. and Atala, A. 2007. Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotech. 25(1): 100-106.
Mostoslavsky G., Fabian, A.J., Rooney, S., Alt, F.W. and Mulligan, R.C. 2006. Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer. PNAS. 103(44): 16406-16411.
Murphy, G.J., Mostoslavsky, G., Kotton, D.N. and Mulligan R.C. 2006. Exogenous control of mammalian gene expression via modulation of translational termination. Nat. Medicine. 12(9): 1093-1099.
Mostoslavsky, R., Chua, K.F, Lombard, D.L., Pang, W.W, Fischer, M.R., Gellon, L., Liu, P., Mostoslavsky, G., Franco, S., Murphy, M.M., Mills, K.D., Patel, P., Hsu, J., Hong, A.L., Ford, E., Cheng, H-L., Kennedy, C., Nunez, N., Bronson, R., Frendewey, D., Auerbach, W., Valenzuela, D., Karow, M., Hottiger, M.O., Hursting, S., Barrett, J.C., Guarente, L., Mulligan, R., Demple, B., Yancopolous, G.D., and Alt, F.W. 2006. Genomic instability and aging-like phenotype in the absence of mammalian SIRT6. Cell. 124:1-15.
Mostoslavsky, G., Kotton, D.N., Fabian, A.J., Lee, J-S, Gray, J.T., and Mulligan, R.C. 2005. Efficiency of transduction of highly purified murine hematopoietic stem cells by lentiviral and oncoretroviral vectors under conditions of minimal in vitro manipulation. Mol. Therapy. 11 (6): 932-940.
Technologies available for sharing upon request:
Production of retroviral and lentiviral vectors for gene transfer Reprogramming of somatic cells into induced Pluripotent Stem Cells (iPS); Purification of Hematopoietic Stem Cells; Transduction of all type of Stem Cells; Differentiation of embryonic stem cells (ESC) and iPS cells towards definitive endoderm