Single Cell Sequencing Core

Boston University’s Single Cell Sequencing Core provides in-house services for single cell RNA sequencing to all researchers at Boston University and to external researchers on an available-resource basis. As a high-throughput core, we provide an array of services to get you from sample to high-quality single cell gene expression data and analysis. We offer multiple single cell library preparation options and next-generation sequencing.

Our single cell services include library preparation using microfluidic devices using the Chromium Single Cell 3′ System from 10x Genomics and Bio-Rad® Single-Cell ddSEQ Single-Cell Isolator, or manual plate-based library preparations using CEL-Seq2. We interface with the BUMC Microarray and Sequencing Core to perform Illumina sequencing of single cell libraries. We also perform tissue dissociation and Bioinformatic analysis as a fee-for-service. Visit our services section for more information. 

Single Cell Library/ Application

Input Cell Concentration

Captured Cells

Library Preparation

Chromium 10x Genomics

100-2,000 cells/uL 500- >10,000 cells $1,650 per sample
(4 samples min)

ddSEQ Single Cell Isolator

2,500 cells/uL 300-500 cells $475 per sample
(4 samples min)

CEL-seq

——— < 96 cells $550 per plate

 

Application

Internal Pricing

External Pricing

Midi Output – 130M Single Reads or 260M Paired End Reads

150 Cycles $1,450 $1,550
300 Cycles $2,050 $2,150

High Output – 400M Single Reads or 800M Paired End Reads

75 Cycles $1,800 $1,900
150 Cycles $3,050 $3,150
300 Cycles $4,550 $4,675

Please submit a consultation request form before submitting your services on our submission form.

Contact

Yuriy Alekseyev
Single Cell Sequencing Core Director 
Boston University School of Medicine
75 East Newton St., Evans Building 6th Floor
Boston MA 02118
(617) 414-1369

E-mail: Single Cell Sequencing Core Lab