Carl Zeiss LSM 710 NLO Microscope System

zeiss lsm 710 mlo
Zeiss LSM 710 NLO

The Carl Zeiss LSM 710 NLO microscope system equipped with 6 visible laser lines (458nm, 488nm, 514nm, 561nm, 594nm, 633nm), a 405nm solid state laser as well as a tunable femtosecond IR pulse laser for multiphoton excitation (Coherent Chameleon Vision–S).

In addition to two descanned internal detectors and a spectral detector which enables lambda scanning and linear unmixing of fluorescent probes, the system has two highly sensitive gallium arsenide non-descanned detectors for collection of emitted light from two-photon experiments. The laser scanning system is coupled to an upright microscope with a motorized stage and is complete with a variety of objective lenses (10X, 20X, 40X, 63X).

Applications of the system include:

  • Optical sectioning of fluorescent tissue using confocal or multiphoton imaging
  • Time-lapse imaging of living tissue or cell culture
  • Tile scanning and stitching of composite images
  • 3D reconstruction of biological tissue/cells
  • In vivo time-lapse imaging
  • FRAP, FRET, Bleaching

Location and Availability

BUSM – Instructional Building L-816
Hours of Operation: 24/7
Technician: available by appointment

BU User Rates

$38/hour unassisted
$75/hour assisted
$75/hour training

External User Rates

$75/hour unassisted
$129/hour assisted
$129/hour training

Please use the Cores Equipment Scheduler (CES) for scheduling, pricing and appointment information. For new users click here.

Contact

Tarik F. Haydar, PhD
Core Director
Email: thaydar@bu.edu
Office: (617) 638-4249

Multi-photon Microscope Core Facility