Zeiss LSM 700 Microscope

ZEISS-LSM-700-Confocal-Lase
The Zeiss LSM 700 is equipped with four solid state lasers (405, 488, 555, and 639 nm). Available objectives include 10X dry, 20X dry, 40X oil, and 63X oil. Features of the Zeiss ZEN software include single image acquisition, z-stacks, tiling, programming of multiple locations, time-lapse imaging, and fluorescence recovery after photobleaching (FRAP). Using the proprietary Zeiss software (ZEN) or freeware such as ImageJ or FIJI, one can assess intensity values within regions of interests (ROIs), create 3-D projections from z-stacks, generate histograms, and analyze images for co-localization.

BUMC Rates
  • $22/hour unassisted
  • $80/hour training/assisted
External Academic Users Rates
  • $50/hour unassisted
  • $100/hour training/assisted
External Commercial Users Rates
  • $100/hour unassisted $140/hour training/assisted

Usage

Persons are trained and then evaluated before working independently. Training usually requires about four hours, divided between two sessions. In some cases both parties decide that it is better for the imaging to be performed in an assisted manner. Sign up may be done by physically coming to L904 or emailing either vickery@bu.edu orĀ kkneer@bu.edu for time.

Contact

Vickery Trinkaus-Randall, PhD
Core Director
Tel: (617) 638-5099
Email: vickery@bu.edu

Krisandra Kneer
Tel: (617) 638-4536
Email: kkneer@bu.edu

Locations

  • BUSM Instructional Building/(L building)Room L904
  • BUSM Housman Medical Research Center / (R Building)Room R918

Confocal Microscope Facility