IVIS Capabilities & Publications

Boston University’s IVIS Spectrum has the ability to detect both bioluminescence and fluorescence in vivo. Researchers from many departments of the Medical Campus have been using the camera for their experiments. See below to view their research:

 

1) NOD/SCID mouse fat pad injected with HS578T cell line, pre-labeled with lentiviral dsRed-IRES-mTomato vector; red fluorescence image capture is shown below:

2) BALB/c purified mouse T cells were labeled with Invitrogen QDot 565 nanocrystals and injected into recipient mice via the tail vein. IL-16 was administered intratracheally. Flourescent QDOT image capture at 24 hours is shown below.  (Courtesy Jillian Richmond)

IL16 saline

3) In vivo effects of BMP2 shRNA inhibition on bone formation after marrow ablation. Seen below are In vivo measurements of bioluminescent intensities from mice injected in the tibia with lentivirus overexpressing luciferase (lenti-Luc) compared to PBS injected controls, shown right.   (Courtesy Manish Bais, PhD)

PBS day 3 LV

Selected Publications with BU’s IVIS

Kumbrink J, de la Cueva A, Soni S, Sailer N, Kirsch KH. A truncated phosphorylated p130Cas substrate domain is sufficient to drive breast cancer growth and metastasis formation in vivo. Tumor Biol. 2016 Feb 11. Read Publication.

Alsulaiman M, Bais MV, Trackman PC. Lysyl oxidase propeptide stimulates osteoblast and osteoclast differentiation and enhances PC3 and DU145 prostate cancer cell effects on bone in vivo. J Cell Commun Signal. 2016 Mar;10(1):17-31. doi: 10.1007/s12079-015-0311-9. Epub 2015 Dec 1. Read Publication.

Ozdener GB, Bais MV, Trackman PC. Determination of cell uptake pathways for tumor inhibitor lysyl oxidase propeptide. Mol Oncol. 2016 Jan;10(1):1-23. doi: 10.1016/j.molonc.2015.07.005. Epub 2015 Aug 6. Read Publication.

Bais MV, Kukuruzinska M, Trackman PC. Orthotopic non-metastatic and metastatic oral cancer mouse models. Oral Oncol. 2015 May;51(5):476-82. doi: 10.1016/j.oraloncology.2015.01.012. Epub 2015 Feb 11. Read Publication.

Richmond, M. Finkel, A. Studwell, F. Little, and W. Cruikshank (2011). Introduction of Pro-interleukin-16 inhibits T lymphoblastic leukemia growth in mice. Journal of Cancer Research and Clinical Oncology. Epub. Read Publication

Wilson, AA, Murphy, GJ, Hamakawa, H, Kwok, LW, Srinivasan, S, Hovav, AH, Mulligan, RC, Amar, S, Suki, B and Kotton DN (2010). Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. J Clin Inv 120(1):379–389. Read Publication.

Bais MV, Wigner N, Young M, Toholka R, Graves DT, Morgan EF, Gerstenfeld LC, Einhorn TA (2009) BMP2 is essential for post natal osteogenesis but not for recruitment of osteogenic stem cells. Bone. 45(2): 254-66. Read Publication.