Protein Array Service

Protein/Biomarker/Cytokine Assays, Arrays, Analysis and ELISA Services Offered!

Please feel free to download our Protein Array Service Booklet here for more information about some of the other services we offer!

Multiplexing Using Luminex^® XMAP Technology
We have expanded our Array Service Facility to provide a service for analyzing proteins and cytokines in a single assay format using the Luminex Magpix system. Luminex’s xMAP Technology is based on micron sized color-coded magnetic beads (microspheres) that can be divided into 500 distinct sets. Each bead set is coated with a special mixture of dyes that is unique to a particular bioassay, which allows for the capture and detection of specific analytes from a sample. Inside of the Magpix System an LED light source excites the internal dyes that identify each microsphere particle. A reporter dye is also captured during the analysis via a CCD camera that is used to quantify each biomarker. The Magpix allows for multiplexing of up to 50 unique analytes in a single well on a 96-well plate. Simply bring your samples (sera, supernatant, urine, tears, etc.) and we will run the assays and provide you with quantitative data (pg/ml) for each cytokine or biomarker.

Full Service*

Internal Rate: $495 per plate
External Rate: $853.80 per plate**

• Available for magnetic bead based assays only
• AIC prepares samples and performs assay, acquires data, and provides basic data analysis***
• AIC will order all kits and supplies needed
• Please email or call the AIC to set up a consultation

*Does not include the cost of the kit(s) needed
**Total Costs are also subject to a 15% administrative fee
***Additional data analysis is optional for an extra charge

Assisted Acquisition Only Service

Internal Rate: $175 per plate
External Rate: $311.50 per plate*

• Available for magnetic bead based assays only
• Investigator performs assay and delivers plate to AIC
• AIC will run the plate on the Magpix instrument and provide basic data analysis**
• Investigator should provide AIC with kit documents, sample list in text format and sample layout information

*Total Costs are also subject to a 15% administrative fee
**Additional data analysis is optional for an extra charge

Unassisted Acquisition Only Service ($100 per plate)

Internal Rate: $100 per plate
External Rate: $178 per plate*

• Available for magnetic bead based assays only
• Investigator performs assay on their own
• User acquires data on the Magpix instrument
• User will setup plate configuration and sample information using the Magpix software
• User analyzes their own data*Total Costs are also subject to a 15% administrative fee

Example of Some Cataloged Kits Available:

There are over 110 cataloged arrays available from various manufacturers. Arrays are also available for a wide variety of species including human, rat, mouse, porcine, primate and bovine!

Curiox Biosystems DropArray Platform

How Does it Work?

The DropArray series of microplates consist of a hydrophobic planar surface with 96- or 384- hydrophilic features arrayed in the standard SBS/ANSI format. Assays are prepared in 5–20 µl or 2–4 µl volumes depending on the plate format and reside as a drop on a hydrophilic spot on the plate surface.

DA-Bead Plate

Miniaturizing multiplex Luminex^® assays on DA-Bead plates is simple. The assay is set up with the same cocktail of bead and sample, but the total volume is reduced by 5-fold. This leads to a reduction in total assay reagent cost by >60%. Overall the workflow remains unchanged. Plate washing is performed using the LT210 Washing Station and avoids unnecessary clumping, so that bead consumption is maximized.

DA-Cell plate and DA-Bead plate

For More Information please feel free to visit Curiox’s Website or directly contact us using the information below!

Full service*

Internal Rate: $780 per plate
External Rate: $1,388.40 per plate**

• AIC prepares samples and performs assay using the DA-Bead Plate and Washer, acquires data, and provides basic data analysis**
• Available for magnetic bead based assays only
• AIC will order all kits and supplies needed
• Please email or call the AIC to set up a consultation

*Does not include the cost of the kit(s) needed
**Total Costs are also subject to a 15% administrative fee
***Additional data analysis is optional for an extra charge

Research Services Agreement

The AIC requires that all experiments conducted adhere to IBC guidelines. In addition, all parties must read and sign the Research Services Agreement, which outlines the terms and conditions upon which the Core User and BU-AIC core engage in to conduct the study.

How to Schedule

Please login to iLab system to schedule equipment time or services. For new users please follow the steps outlined in Information for New Users.

Contact Information

For further information on our Analytical Services or to schedule a meeting to develop a project, please contact:

Matthew Au
Associate Director of the AIC
AIC Lab Manager
Boston University Chobanian & Avedisian School of Medicine
650 Albany Street, Room 830
Boston MA 02118
(617) 358-6363 | mau115@bu.edu

Lynn Deng
Director of Analytical Instrumentation Core
650 Albany Street, Room 848
Boston MA 02118
(617) 358-6516 | lynndeng@bu.edu

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