Our laboratory is interested in the wound response and repair of epithelium, neurons and extracellular matrix. 1. In the healthy cornea there are no blood vessels and thus the tissue provides an excellent model to study the communication between nerves and epithelium following injury. Primary neurons are co-cultured with epithelial cells to ask how signaling pathways communicate. These include but are not limited to glutamatergic and purinergic receptor-mediated calcium transients. 2. In addition to these signaling events we have found that when epithelial cells are injured nucleotides are released immediately and cause a distinct phosphorylation of EGF receptor residues that ultimately affect the ability of a cell to migrate. 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. Our experiments are conducted using a number of organ culture and cell models and use biochemical, cell and molecular techniques. 4. Last but not least we are interested in the role of long sugar chains or glycosaminoglycans on the formation of oligomers and fibrils in light chain amyloid disease using atomic force microscopy, negative staining and biochemical technologies.
- Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Division of Graduate Medical Sciences
- Professor, Ophthalmology, Boston University School of Medicine
- University of Wisconsin-Madison, PhD
- Kenyon College, BA
- Published on 2/27/2018
Lee A, Karamichos D, Onochie OE, Hutcheon AEK, Rich CB, Zieske JD, Trinkaus-Randall V. Hypoxia modulates the development of a corneal stromal matrix model. Exp Eye Res. 2018 Feb 27; 170:127-137. PMID: 29496505.
- Published on 5/31/2017
Barrios J, Patel KR, Aven L, Achey R, Minns MS, Lee Y, Trinkaus-Randall VE, Ai X. Early life allergen-induced mucus overproduction requires augmented neural stimulation of pulmonary neuroendocrine cell secretion. FASEB J. 2017 Sep; 31(9):4117-4128. PMID: 28566470.
- Published on 9/19/2016
Minns MS, Trinkaus-Randall V. Purinergic Signaling in Corneal Wound Healing: A Tale of 2 Receptors. J Ocul Pharmacol Ther. 2016 Oct; 32(8):498-503. PMID: 27643999.
- Published on 12/10/2015
Minns MS, Teicher G, Rich CB, Trinkaus-Randall V. Purinoreceptor P2X7 Regulation of Ca(2+) Mobilization and Cytoskeletal Rearrangement Is Required for Corneal Reepithelialization after Injury. Am J Pathol. 2016 Feb; 186(2):285-96. PMID: 26683661.
- Published on 7/17/2015
Derricks KE, Trinkaus-Randall V, Nugent MA. Extracellular matrix stiffness modulates VEGF calcium signaling in endothelial cells: individual cell and population analysis. Integr Biol (Camb). 2015 Sep; 7(9):1011-25. PMID: 26183123.
- Published on 8/20/2014
Sanderson J, Dartt DA, Trinkaus-Randall V, Pintor J, Civan MM, Delamere NA, Fletcher EL, Salt TE, Grosche A, Mitchell CH. Purines in the eye: recent evidence for the physiological and pathological role of purines in the RPE, retinal neurons, astrocytes, Müller cells, lens, trabecular meshwork, cornea and lacrimal gland. Exp Eye Res. 2014 Oct; 127:270-9. PMID: 25151301.
- Published on 4/9/2014
Karamichos D, Hutcheon AE, Rich CB, Trinkaus-Randall V, Asara JM, Zieske JD. In vitro model suggests oxidative stress involved in keratoconus disease. Sci Rep. 2014; 4:4608. PMID: 24714342.
- Published on 3/26/2014
Lee A, Derricks K, Minns M, Ji S, Chi C, Nugent MA, Trinkaus-Randall V. Hypoxia-induced changes in Ca(2+) mobilization and protein phosphorylation implicated in impaired wound healing. Am J Physiol Cell Physiol. 2014 May 15; 306(10):C972-85. PMID: 24671101.
- Published on 3/4/2014
Stepp MA, Zieske JD, Trinkaus-Randall V, Kyne BM, Pal-Ghosh S, Tadvalkar G, Pajoohesh-Ganji A. Wounding the cornea to learn how it heals. Exp Eye Res. 2014 Apr; 121:178-93. PMID: 24607489.
- Published on 10/9/2013
Karamichos D, Rich CB, Zareian R, Hutcheon AE, Ruberti JW, Trinkaus-Randall V, Zieske JD. TGF-ß3 stimulates stromal matrix assembly by human corneal keratocyte-like cells. Invest Ophthalmol Vis Sci. 2013 Oct; 54(10):6612-9. PMID: 24022012.
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