Cryo EM Core Facility

The new Cryo-EM Core facility to be located at the Center for Advanced Biochemical Research will be housing a Glacios-2 cryo-TEM (200 kV). The new Glacios-2 cryo-TEM (200 kV) will be delivered in March 2024.  Dr. Esther Bullitt as Core Director will provide technical support along with the full-time technician in charge of day-to-day operation of the facility, to users on sample preparation and the use of data analysis software.

Instrument

The Glacios-2 cryo-TEM (200 kV) is equipped with a bright XFEG electron source, sample autoloader, SelectrisX energy filter, Falcon 4i DED camera and software/hardware for fringe free imaging to allow rapid single particle data collection.

This instrument is capable of obtaining ~2.4Å (and better) for single particle work and collecting tomographic data on thinner specimen. The Glacios-2 will be run with both EPU and SerialEM software. The microscope will be networked to a local server with 60 Tb of temporary data storage for users and will run cryoSPARC Live software for real time data processing during movie acquisition to evaluate samples. A newly designed ThermoFisher Vitrobot Mark 5 grid plunging robot will be available in the core facility for grid preparation and the ambient relative humidity will be controlled to ~20% or lower. Older Vitrobot models will be made available to users with specific needs that may not be met by the Mark 5 instrument.

Contact

Esther Bullitt, PhD
Core Director
(617) 358-8464 │bullitt@bu.edu

Location

Evans Biomedical Research Center (EBRC)
650 Albany Street, First floor
Boston, MA 02118

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