My academic training and research expertise have provided me with an excellent background in chemistry and biochemistry. My research interest involves the identification and characterization of biomolecules such as proteins and glycans using mass spectrometry techniques and utilizing this information to understand biomolecular deregulation in disease models. I have received guidance from international-leaders in glycobiology, mass-spectrometry, and cancer biology, which enabled me to develop expertise covering many bio- analytical techniques, most importantly advanced mass-spectrometry to study glycoconjugates in disease models such as cancer and neurodegenerative disorders. I have ample hands-on experience in modern molecular and cellular techniques. I forayed into colorectal cancer (CRC) research during my doctoral studies at Macquarie University (MQ) under the primary supervision of Dr. Morten Thaysen-Andersen, an internationally recognized researcher in Glycobiology. During my Ph.D., I developed a wide range of expertise to investigate colorectal cancer cell lines and patient tissue samples using various mass spectrometry proteomics and glycomics. I became devoted to make an impact in the field of cancer evident by my scientific contributions to the field, including my frequently cited CRC paper (Sethi MK et al., J Proteome Res, 2014, IF 4.3, 65 citations, Sethi MK et al., J proteomics, 2015, IF 3.5, 45 citation).
After my Ph.D., I was offered a postdoctoral research fellowship at Boston University (BU), MA, with Prof. Joseph Zaia, an internationally acclaimed leader in mass spectrometry (MS) and Glycoscience. Currently I am an Instructor in Prof. Zaia’s lab. At BU, I have learned (and further developed) MS-based techniques of proteoglycans (PGs) and glycosaminoglycans (GAGs) (Raghunathan R, Sethi MK et al., Methods X, 2019) and applied these techniques to disease models in neurodegeneration including Schizophrenia and Alzheimer’s disease as evidenced by my recent publications (e.g., Chen J, Kawamura T, Sethi MK et al., Sci Rep, 2017). My h-index is 8, i10-index is 7, and I have been cited a total of 258 times with year-on-year increases in the citation rate demonstrating my maturing scientific profile (Google Scholar, March 2020). I have taught undergraduates as a laboratory demonstartor during my Ph.D., and I have mentored two undergraduates, one graduate, and one Ph.D student demonstrating my teaching and mentoring abilities. My long-term career objective is to establish an analytical glycobiology research group with a focus on unraveling the molecular mechanisms in disease models.
- Macquarie University, PhD
- Delhi University, MS
- Khalsa College, BS
- Published on 9/20/2023
Downs M, Curran J, Zaia J, Sethi MK. Analysis of complex proteoglycans using serial proteolysis and EThcD provides deep N- and O-glycoproteomic coverage. Anal Bioanal Chem. 2023 Sep 20. PMID: 37728749.
- Published on 6/20/2022
Downs M, Zaia J, Sethi MK. Mass spectrometry methods for analysis of extracellular matrix components in neurological diseases. Mass Spectrom Rev. 2023; 42(5):1848-1875. PMID: 35719114.
- Published on 2/23/2022
Sethi MK, Downs M, Shao C, Hackett WE, Phillips JJ, Zaia J. In-Depth Matrisome and Glycoproteomic Analysis of Human Brain Glioblastoma Versus Control Tissue. Mol Cell Proteomics. 2022 Apr; 21(4):100216. PMID: 35202840.
- Published on 2/2/2022
Downs M, Sethi MK, Raghunathan R, Layne MD, Zaia J. Matrisome changes in Parkinson's disease. Anal Bioanal Chem. 2022 Apr; 414(9):3005-3015. PMID: 35112150.
- Published on 9/15/2020
Raghunathan R, Sethi MK, Klein JA, Zaia J. Proteomics, Glycomics, and Glycoproteomics of Matrisome Molecules. Mol Cell Proteomics. 2019 Nov; 18(11):2138-2148. PMID: 33451368.
- Published on 4/20/2020
Sethi MK, Downs M, Zaia J. Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis. Mol Omics. 2020 08 01; 16(4):364-376. PMID: 32309832.
- Published on 4/17/2020
Muraoka S, DeLeo AM, Sethi MK, Yukawa-Takamatsu K, Yang Z, Ko J, Hogan JD, Ruan Z, You Y, Wang Y, Medalla M, Ikezu S, Chen M, Xia W, Gorantla S, Gendelman HE, Issadore D, Zaia J, Ikezu T. Proteomic and biological profiling of extracellular vesicles from Alzheimer's disease human brain tissues. Alzheimers Dement. 2020 06; 16(6):896-907. PMID: 32301581.
- Published on 10/16/2019
Chatterjee S, Lee LY, Kawahara R, Abrahams JL, Adamczyk B, Anugraham M, Ashwood C, Sumer-Bayraktar Z, Briggs MT, Chik JHL, Everest-Dass A, Förster S, Hinneburg H, Leite KRM, Loke I, Möginger U, Moh ESX, Nakano M, Recuero S, Sethi MK, Srougi M, Stavenhagen K, Venkatakrishnan V, Wongtrakul-Kish K, Diestel S, Hoffmann P, Karlsson NG, Kolarich D, Molloy MP, Muders MH, Oehler MK, Packer NH, Palmisano G, Thaysen-Andersen M. Protein Paucimannosylation Is an Enriched N-Glycosylation Signature of Human Cancers. Proteomics. 2019 11; 19(21-22):e1900010. PMID: 31419058.
- Published on 9/25/2019
Raghunathan R, Sethi MK, Zaia J. On-slide tissue digestion for mass spectrometry based glycomic and proteomic profiling. MethodsX. 2019; 6:2329-2347. PMID: 31660297.
- Published on 8/30/2019
Raghunathan R, Sethi MK, Klein JA, Zaia J. Proteomics, Glycomics, and Glycoproteomics of Matrisome Molecules. Mol Cell Proteomics. 2019 11; 18(11):2138-2148. PMID: 31471497.
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