Our lab’s main interest is the study of molecular mechanics and cellular motility. We employ single molecule fluorescence techniques and laser trapping assays to study: the mechanism and regulation muscle contraction, cell motility, and the regulation of cell shape. Our lab’s investigations of cardiac muscle contraction focus on the effects of cardiomyopathy-causing amino acid substitutions on myosin’s mechanical and enzymatic properties. We are also investigating the nature and regulation of cytoskeletal dynamics in differentiated vascular smooth muscle cells.
- University of Vermont, PhD
- University of Massachusetts Lowell, MS
- University of Massachusetts Lowell, BS
- Published on 1/21/2023
Halder SS, Rynkiewicz MJ, Creso JG, Sewanan LR, Howland L, Moore JR, Lehman W, Campbell SG. Mechanisms of pathogenicity in the hypertrophic cardiomyopathy-associated TPM1 variant S215L. PNAS Nexus. 2023 Mar; 2(3):pgad011. PMID: 36896133.
- Published on 1/12/2023
Doran MH, Rynkiewicz MJ, Rassici D, Bodt SML, Barry ME, Bullitt E, Yengo CM, Moore JR, Lehman W. Conformational changes linked to ADP release from human cardiac myosin bound to actin-tropomyosin. J Gen Physiol. 2023 Mar 06; 155(3). PMID: 36633586.
- Published on 12/2/2022
Doran MH, Rynkiewicz MJ, Pavadai E, Bodt SML, Rasicci D, Moore JR, Yengo CM, Bullitt E, Lehman W. Myosin loop-4 is critical for optimal tropomyosin repositioning on actin during muscle activation and relaxation. J Gen Physiol. 2023 Feb 06; 155(2). PMID: 36459134.
- Published on 7/28/2021
Sewanan LR, Park J, Rynkiewicz MJ, Racca AW, Papoutsidakis N, Schwan J, Jacoby DL, Moore JR, Lehman W, Qyang Y, Campbell SG. Loss of crossbridge inhibition drives pathological cardiac hypertrophy in patients harboring the TPM1 E192K mutation. J Gen Physiol. 2021 09 06; 153(9). PMID: 34319370.
- Published on 12/11/2020
Racca AW, Rynkiewicz MJ, LaFave N, Ghosh A, Lehman W, Moore JR. M8R tropomyosin mutation disrupts actin binding and filament regulation: The beginning affects the middle and end. J Biol Chem. 2020 Dec 11; 295(50):17128-17137. PMID: 33453963.
- Published on 10/5/2020
Racca AW, Rynkiewicz MJ, LaFave N, Ghosh A, Lehman W, Moore JR. M8R tropomyosin mutation disrupts actin binding and filament regulation: The beginning affects the middle and end. J Biol Chem. 2020 12 11; 295(50):17128-17137. PMID: 33020181.
- Published on 7/16/2020
Doran MH, Pavadai E, Rynkiewicz MJ, Walklate J, Bullitt E, Moore JR, Regnier M, Geeves MA, Lehman W. Cryo-EM and Molecular Docking Shows Myosin Loop 4 Contacts Actin and Tropomyosin on Thin Filaments. Biophys J. 2020 08 18; 119(4):821-830. PMID: 32730789.
- Published on 12/14/2019
Sundar S, Rynkiewicz MJ, Ghosh A, Lehman W, Moore JR. Cardiomyopathy Mutation Alters End-to-End Junction of Tropomyosin and Reduces Calcium Sensitivity. Biophys J. 2020 01 21; 118(2):303-312. PMID: 31882250.
- Published on 5/13/2019
Lehman W, Moore JR, Campbell SG, Rynkiewicz MJ. The Effect of Tropomyosin Mutations on Actin-Tropomyosin Binding: In Search of Lost Time. Biophys J. 2019 06 18; 116(12):2275-2284. PMID: 31130236.
- Published on 2/15/2019
Lehman W, Rynkiewicz MJ, Moore JR. A new twist on tropomyosin binding to actin filaments: perspectives on thin filament function, assembly and biomechanics. J Muscle Res Cell Motil. 2020 03; 41(1):23-38. PMID: 30771202.
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