James E. Galagan, PhD
Professor, Biomedical Engineering
Biography
Dr. Galagan’s research is focused on Systems Biology of microbial organisms, specficially Mycobacterium tuberculosis – the causative agent of tuberculosis – and the fungus Neurospora crassa. The Galagan Lab is an interdisciplinary group that combines computational biology, genomics, and experimental lab work. Specific interests and projects include:
• Using a genomics, computational modeling, and experimental systems-identification techniques to reconstruct the gene regulatory network of M. tuberculosis.
• Developing and experimentally validating computational models of microbial metabolism for M. tuberculosis and N. crassa.
• Using high-throughput sequencing and comparative sequence analysis to study the functional evolution of M. tuberculosis.
• Developing computational methods for genomic data analysis (including gene prediction, gene expression analysis, and polymorphism discovery)
• Developing experimental methods for microbial systems identification and analysis, drawing on electrical and mechanical systems identification methods
Other Positions
- Associate Professor, Virology, Immunology & Microbiology, Boston University Chobanian & Avedisian School of Medicine
- Faculty, National Emerging Infectious Disease Lab, Boston University
- Member, Evans Center for Interdisciplinary Biomedical Research, Boston University
- Associate Director of Systems Biology, National Emerging Infectious Disease Lab, Boston University Chobanian & Avedisian School of Medicine
- Associate Director of Microbial Genome Analysis, Broad Institute
- Member, Genome Science Institute, Boston University
Education
- Massachusetts Institute of Technology, PhD
- University of California, Davis, BS
Publications
- Published on 9/21/2024
Lally P, Tierrafría VH, Gómez-Romero L, Stringer A, Collado-Vides J, Wade JT, Galagan JE. A Cryptic Prophage Transcription Factor Drives Phenotypic Changes via Host Gene Regulation. bioRxiv. 2024 Sep 21. PMID: 39345586.
Read at: PubMed - Published on 5/24/2024
Lally P, Gómez-Romero L, Tierrafría VH, Aquino P, Rioualen C, Zhang X, Kim S, Baniulyte G, Plitnick J, Smith C, Babu M, Collado-Vides J, Wade JT, Galagan JE. Predictive Biophysical Neural Network Modeling of a Compendium of in vivo Transcription Factor DNA Binding Profiles for Escherichia coli. bioRxiv. 2024 May 24. PMID: 38826350.
Read at: PubMed - Published on 4/18/2024
Sankar K, Kuzmanovic U, Schaus SE, Galagan JE, Grinstaff MW. Strategy, Design, and Fabrication of Electrochemical Biosensors: A Tutorial. ACS Sens. 2024 May 24; 9(5):2254-2274. PMID: 38636962.
Read at: PubMed - Published on 10/25/2023
Grazon C, Garanger E, Lalanne P, Ibarboure E, Galagan JE, Grinstaff MW, Lecommandoux S. Transcription-Factor-Induced Aggregation of Biomimetic Oligonucleotide-b-Protein Micelles. Biomacromolecules. 2023 Nov 13; 24(11):5027-5034. PMID: 37877162.
Read at: PubMed - Published on 1/20/2023
Muellers SN, Tararina MA, Kuzmanovic U, Galagan JE, Allen KN. Structural Insights into the Substrate Range of a Bacterial Monoamine Oxidase. Biochemistry. 2023 Feb 07; 62(3):851-862. PMID: 36662673.
Read at: PubMed - Published on 5/4/2022
Grazon C, Chern M, Lally P, Baer RC, Fan A, Lecommandoux S, Klapperich C, Dennis AM, Galagan JE, Grinstaff MW. The quantum dot vs. organic dye conundrum for ratiometric FRET-based biosensors: which one would you chose? Chem Sci. 2022 Jun 07; 13(22):6715-6731. PMID: 35756504.
Read at: PubMed - Published on 5/1/2022
Tierrafría VH, Rioualen C, Salgado H, Lara P, Gama-Castro S, Lally P, Gómez-Romero L, Peña-Loredo P, López-Almazo AG, Alarcón-Carranza G, Betancourt-Figueroa F, Alquicira-Hernández S, Polanco-Morelos JE, García-Sotelo J, Gaytan-Nuñez E, Méndez-Cruz CF, Muñiz LJ, Bonavides-Martínez C, Moreno-Hagelsieb G, Galagan JE, Wade JT, Collado-Vides J. RegulonDB 11.0: Comprehensive high-throughput datasets on transcriptional regulation in Escherichia coli K-12. Microb Genom. 2022 May; 8(5). PMID: 35584008.
Read at: PubMed - Published on 4/12/2022
Sankar K, Baer R, Grazon C, Sabatelle RC, Lecommandoux S, Klapperich CM, Galagan JE, Grinstaff MW. An Allosteric Transcription Factor DNA-Binding Electrochemical Biosensor for Progesterone. ACS Sens. 2022 Apr 22; 7(4):1132-1137. PMID: 35412319.
Read at: PubMed - Published on 2/7/2022
Zamani M, Dupaty J, Baer RC, Kuzmanovic U, Fan A, Grinstaff MW, Galagan JE, Klapperich CM. Paper-Based Progesterone Sensor Using an Allosteric Transcription Factor. ACS Omega. 2022 Feb 22; 7(7):5804-5808. PMID: 35224340.
Read at: PubMed - Published on 1/19/2021
Kolluri N, Kamath S, Lally P, Zanna M, Galagan J, Gitaka J, Kamita M, Cabodi M, Lolabattu SR, Klapperich CM. Development and Clinical Validation of Iso-IMRS: A Novel Diagnostic Assay for P. falciparum Malaria. Anal Chem. 2021 02 02; 93(4):2097-2105. PMID: 33464825.
Read at: PubMed
View 85 more publications: View full profile at BUMC