The long-standing interest of my laboratory is the regulation of signaling pathways, including understanding the molecular mechanisms of action of signaling components in normal biological processes and in disease processes. We are currently focused on understanding the cascade of intracellular events that leads to the activation of Wnt/ß-catenin signaling and the biological role of the Wnt/ß-catenin pathway during embryonic development and in disease. We employ three experimental systems: Xenopus laevis, mice, and cultured cell lines and primary cells. We use these systems to gain information on gene functions with the goal to uncover and characterize biochemical and developmental mechanisms that could ultimately be used to prevent Wnt/beta-catenin deregulation in congenital and adult diseases. Our areas of interest are:
1. To characterize the molecular mechanism leading to activation (stabilization and nuclear translocation) of ß-catenin, the key intracellular Wnt component that is activated in many human tumors
2. To define the role of Wnt/ß-catenin signaling during embryonic development, including its role in dorso-ventral axis formation and organ morphogenesis
3. To determine how deregulation of Wnt/ß-catenin components leads to diseases such as cancer and congenital defects
- Boston Medical Center
- Assistant Dean, Diversity & Multicultural Affairs, Boston University School of Medicine
- Member, BU-BMC Cancer Center, Boston University
- Member, Evans Center for Interdisciplinary Biomedical Research, Boston University
- Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Graduate Medical Sciences
- Member, Genome Science Institute, Boston University
- Universidad Autónoma de Madrid, PhD
- University of the Basque Country, MSc
- University of the Basque Country, BS
- Published on 10/10/2019
Schaefer S, Doktor TK, Frederiksen SB, Chea K, Hlavacova M, Bruun GH, Rabjerg M, Andresen BS, Dominguez I, Guerra B. Down-regulation of CK2a correlates with decreased expression levels of DNA replication minichromosome maintenance protein complex (MCM) genes. Sci Rep. 2019 10 10; 9(1):14581. PMID: 31601942.
- Published on 6/4/2019
Ann C. Zumwalt, Isabel Dominguez. Integrating the Educators: Outcomes of a Pilot Program to Prime Basic Science Medical Educators for Success in Integrated Curricula. Medical Science Educator. 2019; 29(3):637–642.
- Published on 4/4/2019
Marivin A, Morozova V, Walawalkar I, Leyme A, Kretov DA, Cifuentes D, Dominguez I, Garcia-Marcos M. GPCR-independent activation of G proteins promotes apical cell constriction in vivo. J Cell Biol. 2019 05 06; 218(5):1743-1763. PMID: 30948426.
- Published on 12/4/2017
Chua MMJ, Lee M, Dominguez I. Cancer-type dependent expression of CK2 transcripts. PLoS One. 2017; 12(12):e0188854. PMID: 29206231.
- Published on 11/13/2017
Leyme A, Marivin A, Maziarz M, DiGiacomo V, Papakonstantinou MP, Patel PP, Blanco-Canosa JB, Walawalkar IA, Rodriguez-Davila G, Dominguez I, Garcia-Marcos M. Specific inhibition of GPCR-independent G protein signaling by a rationally engineered protein. Proc Natl Acad Sci U S A. 2017 11 28; 114(48):E10319-E10328. PMID: 29133411.
- Published on 4/29/2017
Lee M, Chea K, Pyda R, Chua M, Dominguez I. Comparative Analysis of Non-viral Transfection Methods in Mouse Embryonic Fibroblast Cells. J Biomol Tech. 2017 Jul; 28(2):67-74. PMID: 28507462.
- Published on 1/28/2017
Chua MM, Ortega CE, Sheikh A, Lee M, Abdul-Rassoul H, Hartshorn KL, Dominguez I. CK2 in Cancer: Cellular and Biochemical Mechanisms and Potential Therapeutic Target. Pharmaceuticals (Basel). 2017 Jan 28; 10(1). PMID: 28134850.
- Published on 4/12/2016
Marivin A, Leyme A, Parag-Sharma K, DiGiacomo V, Cheung AY, Nguyen LT, Dominguez I, Garcia-Marcos M. Dominant-negative Ga subunits are a mechanism of dysregulated heterotrimeric G protein signaling in human disease. Sci Signal. 2016 Apr 12; 9(423):ra37. PMID: 27072656.
- Published on 7/1/2015
Apfel J, Parikh JR, Reischmann P, Ewing RM, Müller O, Xia Y, Dominguez I. Systems Biology of Cancer Editor: Sam Thiagalingam. The Wnt signaling network in Cancer. Cambridge University Press. 2015.
- Published on 7/1/2015
Ortega C, Prince-Wright L, Dominguez I. Proceedings of the CK2-VII meeting. Editors Khalil Ahmed, Olaf Issinger and Ryszard Szyska. Role of CK2 in organ formation. 2015.
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