Research in our lab focuses on characterizing the molecular details of protein-ligand and protein-protein interactions and the mechanisms by which changes in these interactions regulate physiological processes. These studies have concentrated mainly on the role of the calcium ion in mediating changes in protein-ligand interactions which subsequently may alter protein-protein interactions to modulate function. However, other protein-ligand interactions are also under study including enzyme-effector and protein-protein complexes. X-ray crystallography is a major tool used in these studies.
- University of Birmingham, PhD
- University of Manchester, BS
- Published on 7/31/2017
Rynkiewicz MJ, Wu H, Cafarella TR, Nikolaidis NM, Head JF, Seaton BA, McCormack FX. Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop. Biochemistry. 2017 Aug 08; 56(31):4095-4105. PMID: 28719181.
- Published on 7/11/2013
Farelli JD, Gumbart JC, Akey IV, Hempstead A, Amyot W, Head JF, McKnight CJ, Isberg RR, Akey CW. IcmQ in the Type 4b secretion system contains an NAD+ binding domain. Structure. 2013 Aug 6; 21(8):1361-73. PMID: 23850453.
- Published on 5/17/2013
Duval V, McMurry LM, Foster K, Head JF, Levy SB. Mutational analysis of the multiple-antibiotic resistance regulator MarR reveals a ligand binding pocket at the interface between the dimerization and DNA binding domains. J Bacteriol. 2013 Aug; 195(15):3341-51. PMID: 23687277.
- Published on 6/8/2012
van Eijk M, Rynkiewicz MJ, White MR, Hartshorn KL, Zou X, Schulten K, Luo D, Crouch EC, Cafarella TR, Head JF, Haagsman HP, Seaton BA. A unique sugar-binding site mediates the distinct anti-influenza activity of pig surfactant protein D. J Biol Chem. 2012 Aug 3; 287(32):26666-77. PMID: 22685299.
- Published on 8/24/2011
Platonova O, Akey IV, Head JF, Akey CW. Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos. Biochemistry. 2011 Sep 20; 50(37):8078-89. PMID: 21863821.
- Published on 11/3/2010
Shang F, Rynkiewicz MJ, McCormack FX, Wu H, Cafarella TM, Head JF, Seaton BA. Crystallographic complexes of surfactant protein A and carbohydrates reveal ligand-induced conformational change. J Biol Chem. 2011 Jan 7; 286(1):757-65. PMID: 21047777.
- Published on 7/2/2010
Hartshorn KL, White MR, Rynkiewicz M, Sorensen G, Holmskov U, Head J, Crouch EC. Monoclonal antibody-assisted structure-function analysis of the carbohydrate recognition domain of surfactant protein D. Am J Physiol Lung Cell Mol Physiol. 2010 Sep; 299(3):L384-92. PMID: 20601494.
- Published on 7/1/2010
Hartshorn KL, White MR, Smith K, Sorensen G, Kuroki Y, Holmskov U, Head J, Crouch EC. Increasing antiviral activity of surfactant protein d trimers by introducing residues from bovine serum collectins: dissociation of mannan-binding and antiviral activity. Scand J Immunol. 2010 Jul; 72(1):22-30. PMID: 20591072.
- Published on 4/27/2010
Seaton BA, Crouch EC, McCormack FX, Head JF, Hartshorn KL, Mendelsohn R. Review: Structural determinants of pattern recognition by lung collectins. Innate Immun. 2010 Jun; 16(3):143-50. PMID: 20423923.
- Published on 4/21/2009
Crouch E, Hartshorn K, Horlacher T, McDonald B, Smith K, Cafarella T, Seaton B, Seeberger PH, Head J. Recognition of mannosylated ligands and influenza A virus by human surfactant protein D: contributions of an extended site and residue 343. Biochemistry. 2009 Apr 21; 48(15):3335-45. PMID: 19249874.
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