The manner in which a cell responds to many growth factor stimuli depends on interactions between glycosaminoglycans (GAGs), growth factors, and growth factor receptors. Extracellular matrix GAGs binds growth factors, creating morphogens gradients essential to tissue patterning. Because these events depend on the fine structure of the GAG chains present, regulation of GAG biosynthesis is a key factor for understanding normal and disease related cellular growth
The key to exploiting an understanding of GAG structure-function relationships for human disease therapy is to winnow oligosaccharide-protein binding patterns from heterogeneous biological preparations. Toward this end, we have developed mass spectral methods for GAGs that enable comparison of structures as a function of biological variables.
The long term research aims are (1) to develop a fundamental understanding of the manner in which glycosaminoglycan expression is varied according to the cellular growth environment related to human disease and (2) to identify HS chain structures useful as therapeutic targets.
New bioinformatics methods are essential to realizing these goals. The data produced using our methods are information rich and not amenable to manual interpretation. Further, the methods needed are distinct from those used in genomics and proteomics. We are developing bioinformatics methods appropriate for interpretation of structural data on glycosaminoglycans and other carbohydrates to identify targets for disease therapy.
- Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Division of Graduate Medical Sciences
- Member, Bioinformatics Graduate Program, Boston University
- Center Faculty Member, Mass Spectrometry, Boston University School of Medicine
- Massachusetts Institute of Technology, PhD
- Bates College, BS
- Published on 6/18/2018
Raghunathan R, Polinski NK, Klein JA, Hogan JD, Shao C, Khatri K, Leon DR, McComb ME, Manfredsson FP, Sortwell CE, Zaia J. Glycomic and Proteomic changes in aging brain nigrostriatal pathway. Mol Cell Proteomics. 2018 Jun 18. PMID: 29915149.
- Published on 6/1/2018
Zaia J, Bierbaum VM. Focus on Mass Spectrometry in Glycobiology and Related Fields, Honoring Catherine E. Costello, Recipient of the 2017 ASMS Award for a Distinguished Contribution in Mass Spectrometry. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1061-1064. PMID: 29855889.
- Published on 5/22/2018
Klein J, Carvalho L, Zaia J. Application of Network Smoothing to Glycan LC-MS Profiling. Bioinformatics. 2018 May 22. PMID: 29790907.
- Published on 5/17/2018
Klein JA, Meng L, Zaia J. Deep sequencing of complex proteoglycans: a novel strategy for high coverage and site-specific identification of glycosaminoglycan-linked peptides. Mol Cell Proteomics. 2018 May 17. PMID: 29773674.
- Published on 5/16/2018
van Eijk M, Rynkiewicz MJ, Khatri K, Leymarie N, Zaia J, White MR, Hartshorn KL, Cafarella TR, van Die I, Hessing M, Seaton BA, Haagsman HP. Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus. J Biol Chem. 2018 Jul 06; 293(27):10646-10662. PMID: 29769321.
- Published on 4/16/2018
Khatri K, Pu Y, Klein JA, Wei J, Costello CE, Lin C, Zaia J. Comparison of Collisional and Electron-Based Dissociation Modes for Middle-Down Analysis of Multiply Glycosylated Peptides. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1075-1085. PMID: 29663256.
- Published on 4/3/2018
Hogan JD, Klein JA, Wu J, Chopra P, Boons GJ, Carvalho L, Lin C, Zaia J. Software for Peak Finding and Elemental Composition Assignment for Glycosaminoglycan Tandem Mass Spectra. Mol Cell Proteomics. 2018 Jul; 17(7):1448-1456. PMID: 29615495.
- Published on 3/21/2018
Wu J, Wei J, Hogan JD, Chopra P, Joshi A, Lu W, Klein J, Boons GJ, Lin C, Zaia J. Negative Electron Transfer Dissociation Sequencing of 3-O-Sulfation-Containing Heparan Sulfate Oligosaccharides. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1262-1272. PMID: 29564812.
- Published on 2/25/2018
Turiák L, Tóth G, Ozohanics O, Révész Á, Ács A, Vékey K, Zaia J, Drahos L. Sensitive method for glycosaminoglycan analysis of tissue sections. J Chromatogr A. 2018 Apr 06; 1544:41-48. PMID: 29506752.
- Published on 2/1/2018
Wu ZL, Person AD, Anderson M, Burroughs B, Tatge T, Khatri K, Zou Y, Wang L, Geders T, Zaia J, Sackstein R. Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology. 2018 Feb 01; 28(2):69-79. PMID: 29186441.
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