G. Graham Shipley, PhD, DSc
Emeritus Professor, Pharmacology, Physiology & Biophysics
Biography
Dr. Shipley is Emeritus Professor of Physiology and Biophysics at Chobanian and Avedisian School of Medicine. His research interests center around the structure and function of cell membranes, receptor-ligand interactions and transmembrane signaling mechanisms. A combination of biochemical, chemical and biophysical approaches are used to probe: (1) membrane lipid conformation, structure, properties and interactions, (2) membrane receptor-lipid interactions and (3) membrane receptor-ligand interactions. Current approaches involve the isolation of membrane-associated proteins and lipids (e.g. low density lipoprotein (LDL) receptor, insulin receptor, integrins, gangliosides), their reconstitution with well characterized lipid monolayer and bilayer matrices and structural studies of the protein-lipid assemblies. Ligand binding studes include LDL receptor/LDL, insulin receptor/insulin, and ganglioside/toxin interactions. The biochemical methods involved include lipid and protein isolation, gel/affinity chromatography, antibody techniques, immunoblotting, detergent solubilization, lipid-protein reconstitution, etc. Chemical methods include lipid sythesis, TLC, HPLC, IR and NMR. The biophysical approach involves x-ray diffraction, protein crystallography, electron microscopy/image reconstruction, surface chemistry and calorimetric and spectroscopic (CD, NMR) methods.
Other Positions
- Graduate Faculty (Primary Mentor of Grad Students), Boston University Chobanian & Avedisian School of Medicine, Graduate Medical Sciences
- Professor, Biochemistry & Cell Biology, Boston University Chobanian & Avedisian School of Medicine
Education
- University of Nottingham, PhD
- University of Nottingham, BSc
Publications
- Published on 9/3/2020
Shipley GG, Tall AR, Atkinson D. In Memoriam: Donald MacFarland Small (1931-2019). J Lipid Res. 2019 May; 60(5):911-912. PMID: 33722375.
Read at: PubMed - Published on 8/24/2009
Doyle DA, Shipley GG. Membranes: editorial overview. Curr Opin Struct Biol. 2009 Aug; 19(4):369-71. PMID: 19709877.
Read at: PubMed - Published on 9/29/2000
Jeon H, Shipley GG. Vesicle-reconstituted low density lipoprotein receptor. Visualization by cryoelectron microscopy. J Biol Chem. 2000 Sep 29; 275(39):30458-64. PMID: 10889196.
Read at: PubMed - Published on 9/29/2000
Jeon H, Shipley GG. Localization of the N-terminal domain of the low density lipoprotein receptor. J Biol Chem. 2000 Sep 29; 275(39):30465-70. PMID: 10889195.
Read at: PubMed - Published on 8/1/2000
Shipley GG. Bilayers and nonbilayers: structure, forces and protein crystallization. Curr Opin Struct Biol. 2000 Aug; 10(4):471-3. PMID: 10981637.
Read at: PubMed - Published on 7/1/2000
Saxena K, Duclos RI, Sripada PK, Shipley GG. Unusual hydration properties of C16:0 sulfatide bilayer membranes. Biophys J. 2000 Jul; 79(1):385-93. PMID: 10866964.
Read at: PubMed - Published on 1/1/2000
Saxena K, Zimmermann P, Schmidt RR, Shipley GG. Bilayer properties of totally synthetic C16:0-lactosyl-ceramide. Biophys J. 2000 Jan; 78(1):306-12. PMID: 10620294.
Read at: PubMed - Published on 12/3/1999
Woldin CN, Hing FS, Lee J, Pilch PF, Shipley GG. Structural studies of the detergent-solubilized and vesicle-reconstituted insulin receptor. J Biol Chem. 1999 Dec 3; 274(49):34981-92. PMID: 10574975.
Read at: PubMed - Published on 5/1/1999
Saxena K, Duclos RI, Zimmermann P, Schmidt RR, Shipley GG. Structure and properties of totally synthetic galacto- and gluco-cerebrosides. J Lipid Res. 1999 May; 40(5):839-49. PMID: 10224153.
Read at: PubMed - Published on 12/16/1997
Saxena K, Shipley GG. Structural studies of detergent-solubilized and vesicle-reconstituted low-density lipoprotein (LDL) receptor. Biochemistry. 1997 Dec 16; 36(50):15940-8. PMID: 9398328.
Read at: PubMed
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