Daniel Cifuentes, PhD

Assistant Professor, Biochemistry

Daniel Cifuentes
(617) 638-4159
72 E. Concord St Silvio Conte (K)

Biography

The beginning of a new life is one of the most enigmatic and dynamic events in Biology. The long term goal of my laboratory is to understand how post-transcriptional regulation of RNA and proteins orchestrates development of a single cell oocyte into a whole embryo and how these mechanisms can be extrapolated to other biological transitions such as cellular reprogramming or malignant transformation.

To address these questions, my laboratory focuses on the mechanisms of post-transcriptional regulation during vertebrate embryogenesis using zebrafish as a model system. This approach allows us to dissect the process of reprogramming in vivo within the context of a whole organism. The combination of fish embryo microinjection together with powerful genetic, genomic, and proteomic approaches provides a unique platform to address these central questions in biology.

Education

  • Universidad de Barcelona, PhD
  • Universidad de Barcelona, BS

Publications

  • Published on 1/1/2018

    Kretov DA, Shafik AM, Cifuentes D. Assessing miR-451 Activity and Its Role in Erythropoiesis. Methods Mol Biol. 2018; 1680:179-190. PMID: 29030849.

    Read at: PubMed
  • Published on 10/3/2016

    Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish. Cold Spring Harb Protoc. 2016 Oct 03; 2016(10):pdb.prot086850. PMID: 27698232.

    Read at: PubMed
  • Published on 10/3/2016

    Vejnar CE, Moreno-Mateos MA, Cifuentes D, Bazzini AA, Giraldez AJ. Optimization Strategies for the CRISPR-Cas9 Genome-Editing System. Cold Spring Harb Protoc. 2016 Oct 03; 2016(10):pdb.top090894. PMID: 27698246.

    Read at: PubMed
  • Published on 1/28/2016

    Hoffman EJ, Turner KJ, Fernandez JM, Cifuentes D, Ghosh M, Ijaz S, Jain RA, Kubo F, Bill BR, Baier H, Granato M, Barresi MJ, Wilson SW, Rihel J, State MW, Giraldez AJ. Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2. Neuron. 2016 Feb 17; 89(4):725-33. PMID: 26833134.

    Read at: PubMed
  • Published on 10/24/2013

    Yoda M, Cifuentes D, Izumi N, Sakaguchi Y, Suzuki T, Giraldez AJ, Tomari Y. Poly(A)-specific ribonuclease mediates 3'-end trimming of Argonaute2-cleaved precursor microRNAs. Cell Rep. 2013 Nov 14; 5(3):715-26. PMID: 24209750.

    Read at: PubMed
  • Published on 10/1/2011

    Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, Wolfe SA. Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct; 138(20):4555-64. PMID: 21937602.

    Read at: PubMed
  • Published on 12/12/2010

    Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D, Curtin SJ, Blackburn JS, Thibodeau-Beganny S, Qi Y, Pierick CJ, Hoffman E, Maeder ML, Khayter C, Reyon D, Dobbs D, Langenau DM, Stupar RM, Giraldez AJ, Voytas DF, Peterson RT, Yeh JR, Joung JK. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nat Methods. 2011 Jan; 8(1):67-9. PMID: 21151135.

    Read at: PubMed
  • Published on 5/6/2010

    Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma E, Mane S, Hannon GJ, Lawson ND, Wolfe SA, Giraldez AJ. A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity. Science. 2010 Jun 25; 328(5986):1694-8. PMID: 20448148.

    Read at: PubMed
  • Published on 3/12/2010

    Pescador N, Villar D, Cifuentes D, Garcia-Rocha M, Ortiz-Barahona A, Vazquez S, Ordoñez A, Cuevas Y, Saez-Morales D, Garcia-Bermejo ML, Landazuri MO, Guinovart J, del Peso L. Hypoxia promotes glycogen accumulation through hypoxia inducible factor (HIF)-mediated induction of glycogen synthase 1. PLoS One. 2010; 5(3):e9644. PMID: 20300197.

    Read at: PubMed
  • Published on 3/24/2008

    Flores E, Cifuentes D, Fernández-Novell JM, Medrano A, Bonet S, Briz MD, Pinart E, Peña A, Rigau T, Rodríguez-Gil JE. Freeze-thawing induces alterations in the protamine-1/DNA overall structure in boar sperm. Theriogenology. 2008 Jun; 69(9):1083-94. PMID: 18359506.

    Read at: PubMed

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