Jeffrey R. Moore, Ph.D.

 Research

Our lab’s main interest is the study of molecular mechanics and cellular motility. We employ single molecule fluorescence techniques and laser trapping assays to study: the mechanism and regulation muscle contraction, cell motility, and the regulation of cell shape. Our lab’s investigations of cardiac muscle contraction focus on the effects of cardiomyopathy-causing amino acid substitutions on myosin’s mechanical and enzymatic properties. We are also investigating the nature and regulation of cytoskeletal dynamics in differentiated vascular smooth muscle cells.

Recent Publications

Farman G. P., Muthu P., Kazmierczak K., Szczesna-Cordary D., Moore J.R. (2014) Impact of Familial Hypertrophic Cardiomyopathy-linked Mutations in the N-Terminus of the RLC on b-Myosin Cross-bridge Mechanics. J. Appl. Physiology. [Epub ahead of print]

Orzechowski M., Moore J.R. Fischer S. Lehman W.L. Farman G. P. (2014) Energy landscapes reveal the myopathic effects of tropomyosin mutations  Arch Biochem Biophys. Dec 15;564:89-99

Guo M., Ehrlicher A.J., Jensen M.H., Renz M., Moore J.R., Goldman R.D., Lippincott-Schwartz J., Mackintosh F.C., Weitz D.A. (2014) Probing the stochastic motor-driven properties of the cytoplasm using force spectrum microscopy. Cell. 158(4):822-832

Huang W, Liang J, Kazmierczak K, Muthu P, Duggal D, Farman GP, Sorensen L, Pozios I, Abraham TP, Moore JR, Borejdo J, Szczesna-Cordary D. (2014) Hypertrophic cardiomyopathy associated Lys104Glu mutation in the myosin regulatory light chain causes diastolic disturbance in mice. J Mol Cell Cardiol. 30;74C:318-329. [Epub ahead of print] PMID: 24992035

Jensen M.H., Morris E.J., Gallant C. M., Morgan K.G., Weitz D.A., Moore J.R. (2013) Mechanism of calponin stabilization of crosslinked actin networks  Biophysical Journal. 106:793-800 PMCID: PMC3944828.

Orzechowski M., Moore J.R. Fischer S. Lehman W.L. (2013) Tropomyosin movement on F-actin during muscle activation explained by energy landscapes.  Arch Biochem Biophys. 545:63-68. PMCID: PMC3943668.

Muthu P., Liang J., Schmidt W., Moore J.R., Szczesna-Cordary D. (2013) In Vitro Rescue Study of a Malignant Familial Hypertrophic Cardiomyopathy Phenotype by Pseudo-Phosphorylation of Myosin Regulatory Light Chain. Arch Biochem Biophys. 552-553: 29-39 PMCID: PMC4043912.

Saphirstein R. J., Gao Y.Z., Jensen M.H., Gallant C.M., Vetterkind S., Moore J.R., Morgan K.G.(2013) The focal adhesion: a regulated component of aortic stiffness. PLoS One. 23;8(4):e62461. doi: 10.1371/journal.pone.0062461. PMCID: PMC3633884.

Guo M., Ehrlicher A.J., Mahammad S., Fabich H., Jensen M.H., Moore J.R., Fredberg J.J., GoldmanR.D., Weitz D.A. (2013) The role of vimentin intermediate filaments in cortical and cytoplasmic mechanics. Biophys J. 2013 105(7):1562-8. PMCID: PMC3791300.

Szczesna-Cordary D., Morimoto S., Gomes A.V., Moore J.R. (2012) Cardiomyopathies:classification, clinical characterization, and functional phenotypes. Biochem Res Int. 870942. PMCID: PMC3529434.

Jensen M.H., Morris E.J., Huang R., Rebowski G., Dominguez R., Weitz D.A., Moore J.R., Wang C-L. (2012) The conformational state of actin filaments regulates branching by actin-related protein 2/3 (Arp2/3) complex. J Biol Chem. 287(37):31447-53 PMCID: PMC3438974

Moore, J. R., Leinwand, L., Warshaw, D. W. (2012) Understanding Cardiomyopathy Phenotypes Based on the Functional Impact of Mutations in the Myosin Motor. Circulation Res. 20;111:375-85.

Jensen M.H., Watt J., Hodgkinson J., Gallant C., Appel S., El-Mezgueldi M., Angelini T., Morgan K.G., Lehman W., and Moore J.R. (2011) Effects of basic calponin on the flexural mechanics and stability of F-actin Cytoskeleton Cytoskeleton 69(1):49-58.PMCID: PMC3355516.

Moore, J.R., Nirody J., Li X.E., Fischer S., Lehman W. (2011) Structural implications of conserved aspartate residues located in tropomyosin’s coiled-coil core. BioArchitecture 1:250-255.PMCID: PMC3384579.

Collins A., Huang R., Jensen M. H., Moore J.R., Lehman W., Wang Chih-Lueh Albert (2011) Structural studies on maturing actin filaments. BioArchitechture. BioArchitechture. 1:127-33.PMCID: PMC3173961.

Lu X., Kazmierczak K., Jiang X.,  Jones M., Watt J., Helfman D., Moore J.R., Szczesna-Cordary D, Lossos D. (2011) Germinal Center Specific Protein HGAL Directly Interacts with Both Myosin and Actin and Increases the Binding of Myosin to Actin FEBS J. 11:1922-31PMCID: PMC3097272

Lin T., Greenberg M.J., Moore J.R., Ostap E.M. (2011) A hearing loss-associated myo1c mutation(R156W) decreases the myosin duty ratio and force sensitivity. Biochemistry. 50(11):1831-8 PMCID: PMC3059334. Pub Med

Greenberg, M. J., Kazmierczak K., Szczesna-Cordary D., Moore J. R.  (2010) Cardiomyopathy-linked myosin regulatory light chain mutations disrupt myosin strain-dependent    biochemistry. Proc Natl Acad Sci U S A. 107(40):17403-8. PMCID: PMC2951453. Pub Med

Greenberg MJ, Moore JR. (2010) The molecular basis of frictional loads in the in vitro motility assay with applications to the study of the loaded mechanochemistry of molecular motors. Cytoskeleton. 67(5):273-85. Pub Med

Greenberg, M., Mealy, T., Jones, M., Szczesna-Cordary, D.,  Moore, J., (2010) The direct molecular effects of fatigue and myosin regulatory light chain phosphorylation on the actomyosin contractile apparatus. Am J Physiol Regul Integr Comp Physiol 298(4):R989-96.PMCID: PMC2853388. Pub Med

Greenberg, M. and Moore, J. (2010) Actin in action and inaction: The differential effects of hypertrophic and dilated cardiomyopathy actin mutations on thin filament J Mol Cell Cardiol. 48:277-8. Pub Med

View full list of publications.

Contact Us

Department of Pharmacology,Physiology & Biophysics
Boston University School of Medicine
700 Albany Street
Boston MA 02118-2526

e-mail: jxmoore@bu.edu
lab website: Moore Laboratory