Selected List of Available Laboratory Protocols
Preparation of murine bone marrow (and megakaryocyte) cultures
Purification of megakaryocytes (gradient-based or by antibody affinity column)
Live Imaging of megakaryocytes
Transfection of primary bone marrow cells
EMSA and foot printing
Blood counts and blood clotting assays
Ploidy determination by Flow Cytometry
FACS Analysis of bone marrow progenitors
Immunohistochemistry
Generation of transgenic constructs
Generation of Knockout constructs
Generation of transgenic and knockout lines
Preparation of mouse or rat aortic vascular smooth muscle cell cultures
Femoral artery injury Model
High Fat Diet induced Atherosclerosis
Cryosection and immunohistochemistry
Mesenchymal stem cell cultures
Differentiation of Bone Marrow Mesenchymal Stem Cells
Figure: β-Gal staining of different organs and cells derived from WT or A2b adenosine receptor knockout, ß-gal knock-in mice (The A2B adenosine receptor protects against inflammation and excessive vascular adhesion. Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. J Clin Invest. 2006 Jul;116(7):1913-23.)
