BU CryoEM Core Facility workflow validation achieves a structure of apoferritin at 2.04 Å!
The BU CryoEM Core Facility validated its Glacios II microscope and Vitrobot plunge-freezing sample preparation device for high-resolution data acquisition by freezing a sample of apoferritin and determining its structure to a resolution of 2.04 Å from a dataset of 930 exposures. The core facility’s external processing workstation running cryoSPARC LIVE provided a preliminary structure by processing data as it was being collected on the microscope. This fully integrated core facility test going from sample to structure validates the BU CryoEM Core Facility for user operation and high-quality data collection. Please reach out to us if you would like to receive training and reserve facility instrumentation.
