Useful experimental models

Examples:

GFP-labeled DNA of Megakaryocytes in vivo:

An in vivo model with GFP-Histone labeled megakaryocytes.

Direct visualization of the endomitotic cell cycle in living megakaryocytes: differential patterns in low and high ploidy cells.

Papadantonakis N, Makitalo M, McCrann DJ, Liu K, Nguyen HG, Martin G, Patel-Hett S, Italiano JE, Ravid K. Cell Cycle. 2008 Aug;7(15):2352-6. Epub 2008 May 21.

To view megakaryocyte endomitosis by live imaging go to:

16NMK

16NMk phase

4-8NMKphase

4-8NMK

2N-4NMKphase

2N-4NMK gfp

A Transgenic Mouse Model:

Conditional expression of genes in the megakaryocyte lineage in vivo.

Nguyen HG, Yu G, Makitalo M, Yang D, Xie HX, Jones MR, Ravid K. Conditional overexpression of transgenes in megakaryocytes and platelets in vivo.Blood. 2005 Sep 1;106(5):1559-64. Epub 2005 May 12.

A Knock Out Mouse Model:

The first A2b adenosine receptor knock out mouse model, also with b-galactosidase knocked in instead of the deleted gene. This model has proven to be crucial for exploring the role of this receptor in inflammation, atherosclerosis and tissue regeneration.

Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. The A2B adenosine receptor protects against inflammation and excessive vascular adhesion.J Clin Invest. 2006 Jul;116(7):1913-23.

mouse model graphic

cindy

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August 6, 2010
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