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Selected List of Available Laboratory Protocols

(upon inquiry, remac@bu.edu)

Preparation of murine bone marrow (and megakaryocyte) cultures

Purification of megakaryocytes (gradient-based or by antibody affinity column)

Live Imaging of megakaryocytes

Transfection of primary bone marrow cells

EMSA and foot printing

Blood counts and blood clotting assays

Ploidy determination by Flow Cytometry

FACS Analysis of bone marrow progenitors


Generation of transgenic constructs

Generation of Knockout constructs

Generation of transgenic and knockout lines

Preparation of mouse or rat aortic vascular smooth muscle cell cultures

Femoral artery injury Model

High Fat Diet induced Atherosclerosis

Cryosection and immunohistochemistry

Mesenchymal stem cell cultures

Differentiation of Bone Marrow Mesenchymal Stem Cells

Figure: β-Gal staining of different organs and cells derived from WT or A2b adenosine receptor knockout, ß-gal knock-in mice (The A2B adenosine receptor protects against inflammation and excessive vascular adhesion. Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. J Clin Invest. 2006 Jul;116(7):1913-23.)


August 19, 2010
Primary teaching affiliate
of BU School of Medicine