Optical Microscopy Cell and Tissue Imaging Cores
The optical microscopy cores at the Boston University Medical campus offer comprehensive, cutting-edge technology and support for a wide range of digital imaging microscopy applications. Located across several facilities, these resources are listed below according to the strengths of each instrument and their supporting facility teams, to better assist you in finding and evaluating the proper equipment and support for your research. These instruments fall into the following categories, best suited for different applications.
Zeiss LSM 880 with Airyscan enables fast and high-resolution.
Suited for live cell and live tissue imaging and tends to penetrate into tissue more efficiently. Femtosecond infrared pulsed laser light is well tolerated by living cells.
Bright-field monochrome and color imaging for immunohistochemistry applications. Fluorescence, for immunofluorescence and expressed fluorescent constructs (fixed or live). With dim samples wide-field coupled with deconvolution software can outperform confocal systems.
Offers faster confocal imaging with generally lower resolution in the Z axis.
Optical sectioning of fluorescent tissue using confocal or multiphoton imaging. Time-lapse imaging of living tissue or cell culture. Tile scanning and stitching of composite images. 3D reconstruction of biological tissue/cells. In vivo time-lapse imaging. FRAP, FRET, Bleaching.
Optical sectioning of fixed and stained cells and tissue. Tile scanning. Time-lapse imaging using wide-field or confocal scanning (environmental chamber available). FRAP. Membrane fluidity measurement. Live cell calcium imaging (flowthrough apparatus available).
Optical sectioning of fixed and stained cells and tissue. Tile scanning. Time-lapse imaging using wide-field or confocal scanning. FRAP.
Low Magnification stereo microscope ideal for imaging live embryos and whole organs. Bright-field monochrome and color as well as fluorescence imaging.
Automated imaging and cell classification and counting in standard multi-well plates. Bright-field and three channel fluorescence cell imaging and identification across the entire well.
General fluorescence fixed and live cell imaging. Imaging of weakly fluorescent samples. Time-lapse live-cell imaging.
Integrated motorized stage allows system to monitor multiple locations over time. Perfect focus system maintains focus for long duration time-lapse imaging.
Point scan confocal imaging using either visible or multiphoton excitation. Time-lapse live cell imaging. Multiple locations can be monitored over time. Images of adjacent regions can be stitched together for increased imaging fields in 2D or 3D. High-speed line scanning available for capturing fast events such as calcium sparks, rapid translocation, and transient membrane potential changes. FRET and FRAP. Photoactivation using visible or multiphoton excitation.
Time lapse of fast events such as protein translocation. Fixed or living samples. DAPI, FITC, Alexa 488, TEXAS red, Alexa 594, Alexa 647, Cy5 and similar indicators. Calcium imaging using Fura 2 (non-confocal). Dual emission for FRET indicators (CFP-YFP).
High-speed ratiometric imaging of intracellular calcium in living cells using Fura 2.
Bright-field color imaging for fixed IHC samples.
Spinning disk confocal and wide-field fluorescence imaging.
Laser point scan confocal with standard and high-speed resonance scanning. Fast AOTF beam splitter for multiple wavelength excitation and emission. High resolution imaging at room temperature. Fast galvo driven Z-stage. 4 channel simultaneous fluorescence imaging.