Ion mobility spectrometry

The MSR is investigating use of IMS-MS for bottom up and top-down applications in the glycosciences. These include glycan isomer analysis, glycoproteomics, and top-down analysis of glycoproteins. The MSR currently houses two mass spectrometers with IMS functionality: a conventional drift-time IMS-Q-TOF instrument, and a dual-source hybrid
Qh-FTICR mass spectrometer equipped with a prototype trapped ion mobility spectrometry (TIMS) device. To learn more about TIMS, click here.

• Baker, E. S.; Burnum-Johnson, K. E.; Ibrahim, Y. M.; Orton, D. J.; Monroe, M. E.; Kelly, R. T.; Moore, R. J.; Zhang, X.; Theberge, R.; Costello, C. E.; Smith, R. D. Enhancing bottom-up and top-down proteomic measurements with ion mobility separations. Proteomics 2015, 15, 2766-76. Pubmed Link
• Pu, Y.; Ridgeway, M. E.; Glaskin, R. S.; Park, M. A.; Costello, C. E.; Lin, C. Separation and Identification of Isomeric Glycans by Selected Accumulation-Trapped Ion Mobility Spectrometry-Electron Activated Dissociation Tandem Mass Spectrometry. Anal. Chem. 2016, 88, 3440-3. Pubmed Link
• Ridgeway, M. E.; Wolff, J. J.; Silveira, J. A.; Lin, C.; Costello, C. E.; Park, M. A. Gated Trapped Ion Mobility Spectrometry Coupled to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Int J Ion Mobil Spectrom 2016, 19, 77-85. Pubmed Link
• Glaskin, R. S.; Khatri, K.; Wang, Q.; Zaia, J.; Costello, C. E. Construction of a Database of Collision Cross Section Values for Glycopeptides, Glycans, and Peptides Determined by IM-MS. Anal. Chem. 2017, 89, 4452-4460. Pubmed Link