Ion mobility spectrometry
The MSR is investigating use of IMS-MS for bottom up and top-down applications in the glycosciences. These include glycan isomer analysis, glycoproteomics, and top-down analysis of glycoproteins. The MSR currently houses two mass spectrometers with IMS functionality: a conventional drift-time IMS-Q-TOF instrument, and a dual-source hybrid Qh-FTICR mass spectrometer equipped with a prototype trapped ion mobility spectrometry (TIMS) device. To learn more about TIMS, click here.
- Baker, E. S.; Burnum-Johnson, K. E.; Ibrahim, Y. M.; Orton, D. J.; Monroe, M. E.; Kelly, R. T.; Moore, R. J.; Zhang, X.; Theberge, R.; Costello, C. E.; Smith, R. D. Enhancing bottom-up and top-down proteomic measurements with ion mobility separations. Proteomics 2015, 15, 2766-76. Pubmed Link
- Pu, Y.; Ridgeway, M. E.; Glaskin, R. S.; Park, M. A.; Costello, C. E.; Lin, C. Separation and Identification of Isomeric Glycans by Selected Accumulation-Trapped Ion Mobility Spectrometry-Electron Activated Dissociation Tandem Mass Spectrometry. Anal. Chem. 2016, . Pubmed Link