Nadezda Sargaeva, Graduate Student
Department of Biochemistry
Mass Spectrometry Resource
Boston University School of Medicine
670 Albany st, Suite 504
Boston, MA 02118
My general research interest is in the study of protein post-translational modifications (PTMs) using tandem MS methods involving ion-electron and ion-ion interactions and similar techniques in high resolving power mass spectrometers such as a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR-MS). I am particularly interested in the identification of isoaspartic acid (isoAsp) resulting from either asparagine (Asn) deamidation or aspartic acid (Asp) isomerization. We have shown in the past that electron capture dissociation (ECD) can generate signature fragments for both Asp and isoAsp. My current research projects include:
(a) Developing other electron based dissociation methods (e.g. electron ionization dissociation, or EID) for Asp/isoAsp differentiation.
(b) Extending these methods to the characterization of other b-amino acid containing peptides, and understanding the fragmentation behavior of these unusual peptides.
(c) Applying these methods to study the isoAsp formation in biologically important systems, such as Amyloid beta protein fragments in Alzheimer patients, including the most toxic form, Ab42.