Expertise on calcium signalling in nerve cells. In most nerve cells, transient increases in intracellular free calcium concentrations (Cai) are caused primarily by influx through voltage-dependent calcium channels. Second messengers like inositol trisphosphate (InsP3) or calcium also have the ability to increase Cai through release from intracellular stores, or gating of calcium channels.
The long-term goal of this laboratory is to investigate mechanisms by which second messengers modulate the excitability of nerve cells by controlling their membrane permeability. We have developed suitable technologies: i) to measure single-channel activities ii) to simultaneously measure changes in intracellular calcium and membrane currents; iii) to pressure-inject pharmacological agents to investigate putative pathways involved in neuronal excitability. The combination of these electrophysiological and pharmacological techniques have proven useful in gathering new and important information about nerve cell function.
There are four main projects:
1. Intracellular calcium regulation and detection in nerve cells. Effects of second messengers on internal calcium and membrane currents in nerve cells.
2. Role of calcium-induced calcium release in the excitability of the peptidergic neurons of Aplysia californica.
3. Role of calcium and Inositol Trisphosphate in phototransduction in Limulus photoreceptors.
4. Genetic Dissociation of phototransduction in Drosophila photoreceptors.
- Graduate Faculty (Primary Mentor of Grad Students), Boston University School of Medicine, Graduate Medical Sciences
- Boston University, PhD
- University of Geneva, MA
- École d'ingénieurs de Genève, BS
- Published on 8/2/2006
Kachoei BA, Knox RJ, Uthuza D, Levy S, Kaczmarek LK, Magoski NS. A store-operated Ca(2+) influx pathway in the bag cell neurons of Aplysia. J Neurophysiol. 2006 Nov; 96(5):2688-98. PMID: 16885525.
- Published on 8/1/2000
Agam K, von Campenhausen M, Levy S, Ben-Ami HC, Cook B, Kirschfeld K, Minke B. Metabolic stress reversibly activates the Drosophila light-sensitive channels TRP and TRPL in vivo. J Neurosci. 2000 Aug 1; 20(15):5748-55. PMID: 10908615.
- Published on 8/1/1996
Levy S, Payne R. Limulus ventral photoreceptors contain two functionally dissimilar inositol triphosphate-induced calcium release mechanisms. J Photochem Photobiol B. 1996 Aug; 35(1-2):97-103. PMID: 8823939.
- Published on 3/1/1994
Fisher TE, Levy S, Kaczmarek LK. Transient changes in intracellular calcium associated with a prolonged increase in excitability in neurons of Aplysia californica. J Neurophysiol. 1994 Mar; 71(3):1254-7. PMID: 8201416.
- Published on 1/1/1993
Levy S, Payne R. A lingering elevation of Cai accompanies inhibition of inositol 1,4,5 trisphosphate-induced Ca release in Limulus ventral photoreceptors. J Gen Physiol. 1993 Jan; 101(1):67-84. PMID: 8436942.
- Published on 6/1/1992
Levy S. Effect of intracellular injection of inositol trisphosphate on cytosolic calcium and membrane currents in Aplysia neurons. J Neurosci. 1992 Jun; 12(6):2120-9. PMID: 1607931.
- Published on 12/6/1988
Levy S, Tillotson D. Effects of Na+ and Ca2+ gradients on intracellular free Ca2+ in voltage-clamped Aplysia neurons. Brain Res. 1988 Dec 6; 474(2):333-42. PMID: 3208137.
- Published on 7/26/1988
Payne R, Walz B, Levy S, Fein A. The localization of calcium release by inositol trisphosphate in Limulus photoreceptors and its control by negative feedback. Philos Trans R Soc Lond B Biol Sci. 1988 Jul 26; 320(1199):359-79. PMID: 2906144.
- Published on 5/1/1987
Levy S, Tillotson D. Ability of the Ca2+-selective microelectrodes to measure fast and local Ca2+ transients in nerve cells. Can J Physiol Pharmacol. 1987 May; 65(5):904-14. PMID: 2441832.
- Published on 8/1/1984
Gorman AL, Levy S, Nasi E, Tillotson D. Intracellular calcium measured with calcium-sensitive micro-electrodes and Arsenazo III in voltage-clamped Aplysia neurones. J Physiol. 1984 Aug; 353:127-42. PMID: 6434727.