Software for Glycomics and Glycoproteomics

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Technical Manual and Documentation
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If you’ve used this software in academic work, please cite: Klein, J., Carvalho, L., & Zaia, J. (2018). Application of Network Smoothing to Glycan LC-MS Profiling. Bioinformatics

GlycReSoft implements algorithms for

  • Generation of Glycan Composition Search Spaces using algebraic rules, the glySpace database network, or from text files
  • Generation of Glycopeptide Sequence Search Spaces using protein sequences in FASTA format or Proteomics search results in PSI mzIdentML format, combined with a glycan search space. This supports N-linked, O-linked, or GAG-linker glycopeptides
  • Deisotoping and charge state deconvolution of glycan and glycopeptide mass spectra
  • Identification and quantification of glycans by MS and glycopeptides by MS/MS

GlycReSoft is able to read LC-MS data in mzML and mzXML format.Though there is currently only a Windows precompiled build, GlycReSoft is platform independent, and is usable from a remote web server, a local desktop computer, or from a terminal.

GlycReSoft is open-source and publicly available. It is made up of several components:
Core Library
Web Application Server
Desktop Application
Deconvolution Library
Glycan Structural Library
Proteomics Library

Here are glycoproteomics test data sets


Glycan Composition LC-MS Search Results Interpretation
Glycopeptide LC-MS/MS Search Results Interpretation
Sample Preprocessing Tutorial

Coming Soon

Glycan Hypothesis Tutorial

More Coming Soon

Glycopeptide Hypothesis Tutorial

Coming Soon

2018 Publication Version (For Review Only)
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