Cryogenic-EM image of detergent extracted Necturus nuclear envelope with NPCs arrayed in local patches as square arrays due to their interactions with the ordered lamina. [Akey, 1989]Cryogenic-EM image: spread Xenopus nuclear envelope with NPCs (high density is black) that exhibit ring-like features in the central channel (black rings) of virtually every particle. [Akey & Radermacher, 1993](top left) 2D average of amphibian NPCs that show a ring-like density in the central channel. (top right, bottom) A cylindrical structure in the central channel of Xenopus NPCs, named the central transporter (in pink), is now understood to be comprised of FG-repeat containing Nups and transport factors. [Akey, 1990; Akey & Radermacher, 1993]
Selected Publications:
Akey CW, Radermacher M. (1993). Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy. J Cell Biol. 122:1-19. doi: 10.1083/jcb.122.1.1. PMID: 8314837; PMCID: PMC2119598.
Akey CW. Visualization of transport-related configurations of the nuclear pore transporter. (1990). Biophys J. 58:341-355. doi: 10.1016/S0006-3495(90)82381-X. PMID: 2207242; PMCID: PMC1280976.
Akey CW. Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy. (1989). J Cell Biol. 109:955-970. doi: 10.1083/jcb.109.3.955. PMID: 2768344; PMCID: PMC2115778.
