Integrated omics from tissue slides

160803 GAG ECM
The interplay of cell surface and extracellular glycoconjugates modulates accessibility of growth factors to cell surface signaling receptors.

Cell surface and extracellular matrix glycoproteins and proteoglycans modulate the accessibility of growth factor ligands to cell surface receptors, thereby influencing signalling transduction.  It is therefore not surprising that dysregulation of homeostasis of cell surface and extracellular matrix molecules is a hallmark of neurological diseases and cancers.  We developed a method for printing glycosidase or protease enzymes to tissue slide surfaces, extraction of the digestion products and LC-MS analysis.

  • Shao, C.; Shi, X.; Phillips, J. J.; Zaia, J. Mass spectral profiling of glycosaminoglycans from histological tissue surfaces. Anal. Chem. 2013, 85, 10984-10991. Pubmed Link
  • Turiák, L.; Shao, C.; Meng, L.; Khatri, K.; Leymarie, N.;Wang, Q.; Pantazopoulos, H.; Leon,

    160803 Integrated omics of TMAs
    A work flow for recovering glycans and proteins

    D. R.; Zaia, J. Workflow for Combined Proteomics and Glycomics Profiling from Histological Tissues. Anal. Chem. 2014, 86, 9670-9678. Pubmed Link