Glycoproteomics

The MSR is developing methods for assignment of site-specific glycosylation using MS and tandem MS. These efforts include development of new tandem MS methods, bioinformatics, and applications to biomedical projects.

Here are our reviews on this topic:ancham_v084i007.indd

  • Leymarie, N.; Zaia, J. Effective use of mass spectrometry for glycan and glycopeptide structural analysis. Anal. Chem. 2012, 84, 3040-8. Pubmed Link
  • Hu, H.; Khatri, K.; Klein, J.; Leymarie, N.; Zaia, J. A review of methods for interpretation of glycopeptide tandem mass spectral data. Glycoconj J 2016, . Pubmed Link
  • Hu, H.; Khatri, K.; Zaia, J. Algorithms and design strategies towards automated glycoproteomics analysis. Mass Spectrom Rev 2016, . Pubmed Link

We organized an intra-laboratory study on assignment of site specific glycoprotein glycosylation:

  • Leymarie, N.; Griffin, P. J.; Jonscher, K.; Kolarich, D.; Orlando, R.; McComb, M.; Zaia, J (corresponding authors) .; Aguilan, J.; Alley, W. R.; Altmann, F.; Ball, L. E.; Basumallick, L.; Bazemore-Walker, C. R.; Behnken, H.; Blank, M. A.; Brown, K. J.; Bunz, S. C.; Cairo, C. W.; Cipollo, J. F.; Daneshfar, R.; Desaire, H.; Drake, R. R.; Go, E. P.; Goldman, R.; Gruber, C.; Halim, A.; Hathout, Y.; Hensbergen, P. J.; Horn, D. M.; Hurum, D.; Jabs, W.; Larson, G.; Ly, M.; Mann, B. F.; Marx, K.; Mechref, Y.; Meyer, B.; Moginger, U.; Neususs, C.; Nilsson, J.; Novotny, M. V.; Nyalwidhe, J. O.; Packer, N. H.; Pompach, P.; Reiz, B.; Resemann, A.; Rohrer, J. S.; Ruthenbeck, A.; Sanda, M.; Schulz, J. M.; Schweiger-Hufnagel, U.; Sihlbom, C.; Song, E.; Staples, G. O.; Suckau, D.; Tang, H.; Thaysen-Andersen, M.; Viner, R. I.; An, Y.; Valmu, L.; Wada, Y.; Watson, M.; Windwarder, M.; Whittal, R.; Wuhrer, M.; Zhu, Y.; Zou, C. Interlaboratory Study on Differential Analysis of Protein Glycosylation by Mass Spectrometry: the ABRF Glycoprotein Research Multi-Institutional Study 2012. Molecular & cellular proteomics : MCP 2013, 12, 2935-2951. Pubmed Link

We developed methods for assigning site specific glycosylation in highly sulfated viral proteins:

  • Crouch, E.; Nikolaidis, N.; McCormack, F.; McDonald, B.; Allen, KMCP

    .; Rynkiewicz, M.; Cafarella, T.; White, M.; Lewnard, K.; Leymarie, N.; Zaia, J.; Seaton, B.; Hartshorn, K. Mutagenesis of SP-D informed by evolution and xray crystallography enhances defenses against Influenza A Virus in vivo. J Biol Chem 2011, 286, 40681-92. Pubmed Link

  • Jiang, J.; Tian, F.; Cai, Y.; Qian, X.; Costello, C. E.; Ying, W. Site-specific qualitative and quantitative analysis of the N- and O-glycoforms in recombinant human erythropoietin. Anal Bioanal Chem 2014, 406, 6265-74. Pubmed Link
  • Khatri, K.; Staples, G. O.; Leymarie, N.; Leon, D. R.; Turi├ík, L.; Huang, Y.; Yip, S.; Hu, H.; Heckendorf, C. F.; Zaia, J. Confident Assignment of Site-Specific Glycosylation in Complex Glycoproteins in a Single Step. J. Proteome Res. 2014, 13, 4347-4355. Pubmed Link
  • Khatri, K.; Klein, J. A.; White, M. R.; Grant, O. C.; Leymarie, N.; Woods, R. J.; Hartshorn, K. L.; Zaia, J. Integrated Omics and Computational Glycobiology Reveal Structural Basis for Influenza A Virus Glycan Microheterogeneity and Host Interactions. Mol Cell Proteomics 2016, 15, 1895-912. Pubmed Link