Glycoproteomics

The MSR is developing methods for assignment of site-specific glycosylation using MS and tandem MS. These efforts include development of new tandem MS methods, bioinformatics, and applications to biomedical projects.

Our manuscript on ExD tandem MS of multiply glycosylated peptides was accepted by JASMS on 1/30/18!

Here are our reviews on glycoproteomics:ancham_v084i007.indd

  • Leymarie, N.; Zaia, J. Effective use of mass spectrometry for glycan and glycopeptide structural analysis. Anal. Chem. 2012, 84, 3040-8. Pubmed Link
  • Hu, H.; Khatri, K.; Klein, J.; Leymarie, N.; Zaia, J. A review of methods for interpretation of glycopeptide tandem mass spectral data. Glycoconj J 2016, 33, 285-296. Pubmed Link

  • Hu, H.; Khatri, K.; Zaia, J. Algorithms and design strategies towards automated glycoproteomics analysis. Mass Spectrom Rev 2017, 36, 475-498. Pubmed Link

We organized an intra-laboratory study on assignment of site specific glycoprotein glycosylation:

  • Leymarie, N.; Griffin, P. J.; Jonscher, K.; Kolarich, D.; Orlando, R.; McComb, M.; Zaia, J (corresponding authors) .; Aguilan, J.; Alley, W. R.; Altmann, F.; Ball, L. E.; Basumallick, L.; Bazemore-Walker, C. R.; Behnken, H.; Blank, M. A.; Brown, K. J.; Bunz, S. C.; Cairo, C. W.; Cipollo, J. F.; Daneshfar, R.; Desaire, H.; Drake, R. R.; Go, E. P.; Goldman, R.; Gruber, C.; Halim, A.; Hathout, Y.; Hensbergen, P. J.; Horn, D. M.; Hurum, D.; Jabs, W.; Larson, G.; Ly, M.; Mann, B. F.; Marx, K.; Mechref, Y.; Meyer, B.; Moginger, U.; Neususs, C.; Nilsson, J.; Novotny, M. V.; Nyalwidhe, J. O.; Packer, N. H.; Pompach, P.; Reiz, B.; Resemann, A.; Rohrer, J. S.; Ruthenbeck, A.; Sanda, M.; Schulz, J. M.; Schweiger-Hufnagel, U.; Sihlbom, C.; Song, E.; Staples, G. O.; Suckau, D.; Tang, H.; Thaysen-Andersen, M.; Viner, R. I.; An, Y.; Valmu, L.; Wada, Y.; Watson, M.; Windwarder, M.; Whittal, R.; Wuhrer, M.; Zhu, Y.; Zou, C. Interlaboratory Study on Differential Analysis of Protein Glycosylation by Mass Spectrometry: the ABRF Glycoprotein Research Multi-Institutional Study 2012. Molecular & cellular proteomics : MCP 2013, 12, 2935-2951. Pubmed Link

We developed methods for assigning site specific glycosylation in highly sulfated viral proteins:

  • Crouch, E.; Nikolaidis, N.; McCormack, F.; McDonald, B.; Allen, KMCP.; Rynkiewicz, M.; Cafarella, T.; White, M.; Lewnard, K.; Leymarie, N.; Zaia, J.; Seaton, B.; Hartshorn, K. Mutagenesis of SP-D informed by evolution and xray crystallography enhances defenses against Influenza A Virus in vivo. J Biol Chem 2011, 286, 40681-92. Pubmed Link
  • Jiang, J.; Tian, F.; Cai, Y.; Qian, X.; Costello, C. E.; Ying, W. Site-specific qualitative and quantitative analysis of the N- and O-glycoforms in recombinant human erythropoietin. Anal Bioanal Chem 2014, 406, 6265-74. Pubmed Link
  • Khatri, K.; Staples, G. O.; Leymarie, N.; Leon, D. R.; Turi├ík, L.; Huang, Y.; Yip, S.; Hu, H.; Heckendorf, C. F.; Zaia, J. Confident Assignment of Site-Specific Glycosylation in Complex Glycoproteins in a Single Step. J. Proteome Res. 2014, 13, 4347-4355. Pubmed Link
  • Khatri, K.; Klein, J. A.; White, M. R.; Grant, O. C.; Leymarie, N.; Woods, R. J.; Hartshorn, K. L.; Zaia, J. Integrated Omics and Computational Glycobiology Reveal Structural Basis for Influenza A Virus Glycan Microheterogeneity and Host Interactions. Mol Cell Proteomics 2016, 15, 1895-912. Pubmed Link