The objective of our research is to establish the detailed structures of biopolymers in order to understand their structure-activity relationships as they influence or reflect biological processes related to health, growth and development, and disease. Our particular focus for new method development is on the needs of glycobiology, since carbohydrates and their conjugates (glycoproteins, glycolipids, etc.) are involved in targeting and immune system recognition, nervous system growth and development, infection, parasite response, carcinogenesis, and other critical processes. The techniques for full structural characterization of these complex molecules are much less developed than are methods for linear biopolymers (proteins and oligonucleotides). Recent introduction of new mass spectral ionization methods and rapid progress in means for mass separation and detection now make it possible to perform structural studies on low picomole amounts of samples even when they are complex mixtures. In our research program we are refining and extending the tools of mass spectrometry and are applying them to studies of biopolymers undertaken with collaborators at BUSM and other institutions around and outside the US. Our laboratory is a Resource Center sponsored by the NIH National Institute of General Medical Sciences.
Cheng Lin – Research Associate Professor
Juan Wei – Postdoctoral Associate (Lin Lab)
Mark McComb – Research Associate Professor
Deborah Francoleon – Research Scientist
Nivedita Bhattacharya- Postdoctoral Associate
Kevin Chandler – Postdoctoral Associate
Yang Tang – Graduate Student (Chemistry)
Vanessa Stahl – UROP student- Biochemistry & Molecular Biology
Angelo Lopez – Research Fellow (Dual appointment with Samuelson Lab at BUGSDM)
Christian Heckendorf – Research Fellow
Abby Gelb – Visiting Researcher (VA Bedford)
Kenichi Ozaki – Visiting Researcher (ONO Pharmaceuticals)
Nickita Mehta – Visiting Researcher (Ragon Institute of MGH, Harvard and MIT)
Shao D, Yao C, Kim MH, Fry J, Cohen RA, Costello CE, Matsui R, Seta F, McComb ME, Bachschmid MM. Improved mass spectrometry-based activity assay reveals oxidative and metabolic stress as sirtuin-1 regulators. Redox Biol. 2019 Mar 05; 22:101150. PMID: 30877853.
Campbell MP, Abrahams JL, Rapp E, Struwe WB, Costello CE, Novotny M, Ranzinger R, York WS, Kolarich D, Rudd PM, Kettner C. The Minimum Information Required for a Glycomics Experiment (MIRAGE) Project: LC Guidelines. Glycobiology. 2019 Feb 19. PMID: 30778580.
Nguyen DN, Xu B, Stanfield RL, Bailey JK, Horiya S, Temme JS, Leon DR, LaBranche CC, Montefiori DC, Costello CE, Wilson IA, Krauss IJ. Oligomannose Glycopeptide Conjugates Elicit Antibodies Targeting the Glycan Core Rather than Its Extremities. ACS Cent Sci. 2019 Feb 27; 5(2):237-249. PMID: 30834312.
Wei J, Wu J, Tang Y, Ridgeway ME, Park MA, Costello CE, Zaia J, Lin C. Characterization and Quantification of Highly Sulfated Glycosaminoglycan Isomers by Gated-Trapped Ion Mobility Spectrometry Negative Electron Transfer Dissociation MS/MS. Anal Chem. 2019 Feb 19; 91(4):2994-3001. PMID: 30649866.
Chandler KB, Mehta N, Leon DR, Suscovich T, Alter G, Costello CE. Multi-Isotype Glycoproteomic Characterization of Serum Antibody Heavy Chains Reveals Isotype- and Subclass-Specific N-Glycosylation Profiles. Mol Cell Proteomics. 2019 Jan 18. PMID: 30659065.
Bandini G, Leon DR, Hoppe CM, Zhang Y, Agop-Nersesian C, Shears MJ, Mahal LK, Routier FH, Costello CE, Samuelson J. O-Fucosylation of thrombospondin-like repeats is required for processing of microneme protein 2 and for efficient host cell invasion by Toxoplasma gondii tachyzoites. J Biol Chem. 2019 Feb 08; 294(6):1967-1983. PMID: 30538131.
Ho RX, Meyer RD, Chandler KB, Ersoy E, Park M, Bondzie PA, Rahimi N, Xu H, Costello CE, Rahimi N. MINAR1 is a Notch2-binding protein that inhibits angiogenesis and breast cancer growth. J Mol Cell Biol. 2018 Jun 01; 10(3):195-204. PMID: 29329397.
Hoppe CM, Albuquerque-Wendt A, Bandini G, Leon DR, Shcherbakova A, Buettner FFR, Izquierdo L, Costello CE, Bakker H, Routier FH. Apicomplexan C-Mannosyltransferases Modify Thrombospondin Type I-containing Adhesins of the TRAP Family. Glycobiology. 2018 05 01; 28(5):333-343. PMID: 29432542.
Khatri K, Pu Y, Klein JA, Wei J, Costello CE, Lin C, Zaia J. Comparison of Collisional and Electron-Based Dissociation Modes for Middle-Down Analysis of Multiply Glycosylated Peptides. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1075-1085.View Related Profiles. PMID: 29663256.
Tang Y, Wei J, Costello CE, Lin C. Characterization of Isomeric Glycans by Reversed Phase Liquid Chromatography-Electronic Excitation Dissociation Tandem Mass Spectrometry. J Am Soc Mass Spectrom. 2018 Jun; 29(6):1295-1307. PMID: 29654534.
Khatri K, Klein JA, Haserick JR, Leon DR, Costello CE, McComb ME, Zaia J. Microfluidic Capillary Electrophoresis-Mass Spectrometry for Analysis of Monosaccharides, Oligosaccharides, and Glycopeptides. Anal Chem. 2017 Jun 06.
Pianta A, Arvikar S, Strle K, Drouin EE, Wang Q, Costello CE, Steere AC. Evidence of the Immune Relevance of Prevotella copri, a Gut Microbe, in Patients With Rheumatoid Arthritis. Arthritis Rheumatol. 2017 May; 69(5):964-975. PMID: 27863183;
Glaskin RS, Khatri K, Wang Q, Zaia J, Costello CE. Construction of a Database of Collision Cross Section Values for Glycopeptides, Glycans, and Peptides Determined by IM-MS. Anal Chem. 2017 Apr 18; 89(8):4452-4460.28323417
Complete list can be found at BU Profiles