Catherine E. Costello

costelloProfessor
Director, Mass Spectrometry Resource

Boston University School of Medicine
Mass Spectrometry Resource
670 Albany Street, Rm 511
Boston, MA 02118-2526

Phone: 617-638-6490
Fax: 617-638-6491
Email: cecmsms@bu.edu

Joint Appointment: Professor, Department of Physiology & Biophysics

Web site for Mass Spectrometry Resource

Education

AB, Emmanuel College, Boston, MA
MS, PhD, Georgetown University, Washington, DC

People

Xiaofeng Shi
Postdoctoral Fellow		 Chunxiang Yao
Postdoctoral Fellow		 Nadezda Sargaeva
Student
Roger Theberge
Postdoctoral Fellow		 Stephen Whelan
Research Scientist		 Xiang Yu
Student
Mengdi Fan
Student		 Ying Zhou
Student
Erica Ebbel
Student		 Yanyan Lu
Student

Research Interests

Biopolymer structural studies based on development and application of mass spectral methods

The objective of our research is to establish the detailed structures of biopolymers in order to understand their structure-activity relationships as they influence or reflect biological processes related to health, growth and development, and disease. Our particular focus for new method development is on the needs of glycobiology, since carbohydrates and their conjugates (glycoproteins, glycolipids, etc.) are involved in targeting and immune system recognition, nervous system growth and development, infection, parasite response, carcinogenesis, and other critical processes. The techniques for full structural characterization of these complex molecules are much less developed than are methods for linear biopolymers (proteins and oligonucleotides). Recent introduction of new mass spectral ionization methods and rapid progress in means for mass separation and detection now make it possible to perform structural studies on low picomole amounts of samples even when they are complex mixtures. In our research program we are refining and extending the tools of mass spectrometry and are applying them to studies of biopolymers undertaken with collaborators at BUSM and other institutions around and outside the US. Our laboratory is a Resource Center sponsored by the NIH National Center for Research Resources.

Research Themes

Neuroscience & Aging; Proteomics & Glycomics

Representative Publications

PubMed

  • Seward, R. J.; Drouin, E. E.; Steere, A. C.; Costello, C. E. Peptides Presented by HLA-DR Molecules in Synovia of Patients with Rheumatoid Arthritis or Antibiotic-Refractory Lyme Arthritis. Mol Cell Proteomics 2011, 10, M110 002477. Pubmed Link
  • Zee, R. S.; Yoo, C. B.; Pimentel, D. R.; Perlman, D. H.; Burgoyne, J. R.; Hou, X.; McComb, M. E.; Costello, C. E.; Cohen, R. A.; Bachschmid, M. M. Redox regulation of sirtuin-1 by S-glutathiolation. Antioxidants & redox signaling 2010, 13, 1023-32. Pubmed Link
  • Wada, Y.; Dell, A.; Haslam, S. M.; Tissot, B.; Canis, K.; Azadi, P.; Backstrom, M.; Costello, C. E.; Hansson, G. C.; Hiki, Y.; Ishihara, M.; Ito, H.; Kakehi, K.; Karlsson, N.; Kato, K.; Kawasaki, N.; Khoo, K. H.; Kobayashi, K.; Kolarich, D.; Kondo, A.; Lebrilla, C.; Nakano, M.; Narimatsu, H.; Novak, J.; Novotny, M. V.; Ohno, E.; Packer, N. H.; Renfrow, M. B.; Tajiri, M.; Thomsson, K. A.; Yagi, H.; Yu, S. Y.; Taniguchi, N. Comparison of Methods for Profiling O-glycosylation: HUPO Human Disease Glycomics/Proteome Initiative Multi-Institutional Study of IgA1. Mol Cell Proteomics 2010, 9, 719-727. Pubmed Link
  • Staples, G. O.; Naimy, H.; Yin, H.; Kileen, K.; Kraiczek, K.; Costello, C. E.; Zaia, J. Improved hydrophilic interaction chromatography LC/MS of heparinoids using a chip with postcolumn makeup flow. Anal. Chem. 2010, 82, 516-22. Pubmed Link
  • Ren, R.; Hong, Z.; Gong, H.; Laporte, K.; Skinner, M.; Seldin, D. C.; Costello, C. E.; Connors, L. H.; Trinkaus-Randall, V. Role of glycosaminoglycan sulfation in the formation of immunoglobulin light chain amyloid oligomers and fibrils. J Biol Chem 2010, 285, 37672-82. Pubmed Link
  • Palaima, E.; Leymarie, N.; Stroud, D.; Mizanur, R. M.; Hodgkin, J.; Gravato-Nobre, M. J.; Costello, C. E.; Cipollo, J. F. The Caenorhabditis elegans bus-2 mutant reveals a new class of O-glycans affecting bacterial resistance. J Biol Chem 2010, 285, 17662-72. Pubmed Link
  • Li, X.; Lin, C.; Han, L.; Costello, C. E.; O’Connor, P. B. Charge remote fragmentation in electron capture and electron transfer dissociation. J Am Soc Mass Spectrom 2010, 21, 646-56. Pubmed Link
  • Ebbel, E. N.; Leymarie, N.; Schiavo, S.; Sharma, S.; Gevorkian, S.; Hersch, S.; Matson, W. R.; Costello, C. E. Identification of phenylbutyrate-generated metabolites in Huntington disease patients using parallel liquid chromatography/electrochemical array/mass spectrometry and off-line tandem mass spectrometry. Anal Biochem 2010, 399, 152-61. Pubmed Link
  • Chatterjee, A.; Carpentieri, A.; Ratner, D. M.; Bullitt, E.; Costello, C. E.; Robbins, P. W.; Samuelson, J. Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. PLoS Pathog 2010, 6, e1001059. Pubmed Link
  • Carpentieri, A.; Ratner, D. M.; Ghosh, S. K.; Banerjee, S.; Bushkin, G. G.; Cui, J.; Lubrano, M.; Steffen, M.; Costello, C. E.; O’Keefe, B.; Robbins, P. W.; Samuelson, J. The anti-retroviral lectin cyanovirin-N targets well-known and novel targets on the surface of Entamoeba histolytica trophozoites. Eukaryot Cell 2010, 9, 1661-1668. Pubmed Link
  • Young, D. C.; Kasmar, A.; Moraski, G.; Cheng, T. Y.; Waltz, A. J.; Hu, J.; Xu, X.; Endres, G. W.; Uzieblo, A.; Zajonc, D.; Costello, C. E.; Miller, M. J.; Moody, D. B. Synthesis of dideoxymycobactin antigens presented by CD1A reveals T cell fine specificity for natural lipopeptide structures. J Biol Chem 2009, 284, 25087-25096. Pubmed Link
  • Ying, W.; Perlman, D. H.; Li, L.; Theberge, R.; Costello, C. E.; McComb, M. E. Highly efficient and selective enrichment of peptide subsets combining fluorous chemistry with reversed-phase chromatography. Rapid Commun Mass Spectrom 2009, 23, 4019-30. Pubmed Link
  • Wang, Y.; Meriin, A. B.; Zaarur, N.; Romanova, N. V.; Chernoff, Y. O.; Costello, C. E.; Sherman, M. Y. Abnormal proteins can form aggresome in yeast: aggresome-targeting signals and components of the machinery. FASEB J 2009, 23, 451-63. Pubmed Link
  • Staples, G.; Bowman, M.; Costello, C. E.; Hitchcock, A.; Lau, J.; Leymarie, N.; Miller, C.; Naimy, H.; Shi, X.; Zaia, J. A Chip-based Amide-HILIC LC/MS Platform for Glycosaminoglycan Glycomics. Proteomics 2009, 9, 686-695. Pubmed Link
  • Singh, B. N.; Hayes, G. R.; Lucas, J. J.; Sommer, U.; Viseux, N.; Mirgorodskaya, E.; Trifonova, R. T.; Sassi, R. R.; Costello, C. E.; Fichorova, R. N. Structural details and composition of Trichomonas vaginalis lipophosphoglycan in relevance to the epithelial immune function. Glycoconj J 2009, 26, 3-17. Pubmed Link
  • Romesser, P. B.; Perlman, D. H.; Faller, D. V.; Costello, C. E.; McComb, M. E.; Denis, G. V. Development of a malignancy-associated proteomic signature for diffuse large B-cell lymphoma. The American journal of pathology 2009, 175, 25-35. Pubmed Link
  • Rex, S.; Beaulieu, L. M.; Perlman, D. H.; Vitseva, O.; Blair, P. S.; McComb, M. E.; Costello, C. E.; Freedman, J. E. Immune versus thrombotic stimulation of platelets differentially regulates signalling pathways, intracellular protein-protein interactions, and alpha-granule release. Thromb Haemost 2009, 102, 97-110. Pubmed Link
  • Perlman, D. H.; Bauer, S. M.; Ashrafian, H.; Bryan, N. S.; Garcia-Saura, M. F.; Lim, C. C.; Fernandez, B. O.; Infusini, G.; McComb, M. E.; Costello, C. E.; Feelisch, M. Mechanistic insights into nitrite-induced cardioprotection using an integrated metabolomic/proteomic approach. Circ Res 2009, 104, 796-804. Pubmed Link
  • Makkinje, A.; Near, R. I.; Infusini, G.; Vanden Borre, P.; Bloom, A.; Cai, D.; Costello, C. E.; Lerner, A. AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern. Cell Signal 2009, 21, 1423-35. Pubmed Link