Catherine E. Costello

Professor

William Fairfield Warren Distinguished Professor

Director, Mass Spectrometry Resource

Boston University School of Medicine
Mass Spectrometry Resource
670 Albany Street, Rm 511
Boston, MA 02118-2526

Phone: 617-638-6490
Fax: 617-638-6491
Email: cecmsms@bu.edu

Joint Appointment: Professor, Department of Physiology & Biophysics

Web site for Mass Spectrometry Resource

Education

AB, Emmanuel College, Boston, MA
MS, PhD, Georgetown University, Washington, DC

People

Xiaofeng Shi Postdoctoral Fellow	Chunxiang Yao Postdoctoral Fellow	Nadezda Sargaeva Student
Roger Theberge Postdoctoral Fellow	Stephen Whelan Research Scientist	Xiang Yu Student
	Mengdi Fan Student	Ying Zhou Student
	Erica Ebbel Student	Yanyan Lu Student

Research Interests

Biopolymer structural studies based on development and application of mass spectral methods

The objective of our research is to establish the detailed structures of biopolymers in order to understand their structure-activity relationships as they influence or reflect biological processes related to health, growth and development, and disease. Our particular focus for new method development is on the needs of glycobiology, since carbohydrates and their conjugates (glycoproteins, glycolipids, etc.) are involved in targeting and immune system recognition, nervous system growth and development, infection, parasite response, carcinogenesis, and other critical processes. The techniques for full structural characterization of these complex molecules are much less developed than are methods for linear biopolymers (proteins and oligonucleotides). Recent introduction of new mass spectral ionization methods and rapid progress in means for mass separation and detection now make it possible to perform structural studies on low picomole amounts of samples even when they are complex mixtures. In our research program we are refining and extending the tools of mass spectrometry and are applying them to studies of biopolymers undertaken with collaborators at BUSM and other institutions around and outside the US. Our laboratory is a Resource Center sponsored by the NIH National Center for Research Resources.

Research Themes

Neuroscience & Aging; Proteomics & Glycomics

Representative Publications

PubMed

• A novel human autoantigen, endothelial cell growth factor, is a target of T and B cell responses in patients with Lyme disease. Drouin EE, Seward RJ, Strle K, McHugh G, Katchar K, Londoño D, Yao C, Costello CE, Steere AC. Arthritis Rheum. 2013 Jan;65(1):186-96.
• β-1,3-glucan, which can be targeted by drugs, forms a trabecular scaffold in the oocyst walls of Toxoplasma and Eimeria. Bushkin GG, Motari E, Magnelli P, Gubbels MJ, Dubey JP, Miska KB, Bullitt E, Costello CE, Robbins PW, Samuelson J. MBio. 2012 Sep 25;3(5).
• Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1. Chinnapen DJ, Hsieh WT, te Welscher YM, Saslowsky DE, Kaoutzani L, Brandsma E, D’Auria L, Park H, Wagner JS, Drake KR, Kang M, Benjamin T, Ullman MD, Costello CE, Kenworthy AK, Baumgart T, Massol RH, Lencer WI. Dev Cell. 2012 Sep 11;23(3):573-86.
• Energy-dependent electron activated dissociation of metal-adducted permethylated oligosaccharides. Yu X, Huang Y, Lin C, Costello CE. Anal Chem. 2012 Sep 4;84(17):7487-94.
• Top-down study of β2-microglobulin deamidation. Li X, Yu X, Costello CE, Lin C, O’Connor PB. Anal Chem. 2012 Jul 17;84(14):6150-7.
• Protective B-cell epitopes of Francisella tularensis O-polysaccharide in a mouse model of respiratory tularaemia. Lu Z, Madico G, Roche MI, Wang Q, Hui JH, Perkins HM, Zaia J, Costello CE, Sharon J. Immunology. 2012 Jul;136(3):352-60.
• A typical preparation of Francisella tularensis O-antigen yields a mixture of three types of saccharides. Wang Q, Shi X, Leymarie N, Madico G, Sharon J, Costello CE, Zaia J. Biochemistry. 2011 Dec 20;50(50):10941-50.
• Oxidation of HRas cysteine thiols by metabolic stress prevents palmitoylation in vivo and contributes to endothelial cell apoptosis. Burgoyne JR, Haeussler DJ, Kumar V, Ji Y, Pimental DR, Zee RS, Costello CE, Lin C, McComb ME, Cohen RA, Bachschmid MM. FASEB J. 2012 Feb;26(2):832-41.
• Electron transfer dissociation of milk oligosaccharides. Han L, Costello CE. J Am Soc Mass Spectrom. 2011 Jun;22(6):997-1013.
• The human proteome project: current state and future direction. Legrain P, Aebersold R, Archakov A, Bairoch A, Bala K, Beretta L, Bergeron J, Borchers CH, Corthals GL, Costello CE, Deutsch EW, Domon B, Hancock W, He F, Hochstrasser D, Marko-Varga G, Salekdeh GH, Sechi S, Snyder M, Srivastava S, Uhlén M, Wu CH, Yamamoto T, Paik YK, Omenn GS. Mol Cell Proteomics. 2011 Jul;10(7):M111.
• Top-Down Analysis of Small Plasma Proteins Using an LTQ-Orbitrap. Potential for Mass Spectrometry-Based Clinical Assays for Transthyretin and Hemoglobin. Théberge R, Infusini G, Tong W, McComb ME, Costello CE. Int J Mass Spectrom. 2011 Mar 1;300(2-3):130-142.
• Chymotrypsin C is a co-activator of human pancreatic procarboxypeptidases A1 and A2. Szmola R, Bence M, Carpentieri A, Szabó A, Costello CE, Samuelson J, Sahin-Tóth M. J Biol Chem. 2011 Jan 21;286(3):1819-27. doi: 10.1074/jbc.M110.187369. Epub 2010 Nov 22
• Seward, R. J.; Drouin, E. E.; Steere, A. C.; Costello, C. E. Peptides Presented by HLA-DR Molecules in Synovia of Patients with Rheumatoid Arthritis or Antibiotic-Refractory Lyme Arthritis. Mol Cell Proteomics2011, 10, M110 002477. Pubmed Link
• Zee, R. S.; Yoo, C. B.; Pimentel, D. R.; Perlman, D. H.; Burgoyne, J. R.; Hou, X.; McComb, M. E.; Costello, C. E.; Cohen, R. A.; Bachschmid, M. M. Redox regulation of sirtuin-1 by S-glutathiolation. Antioxidants & redox signaling2010, 13, 1023-32. Pubmed Link
• Wada, Y.; Dell, A.; Haslam, S. M.; Tissot, B.; Canis, K.; Azadi, P.; Backstrom, M.; Costello, C. E.; Hansson, G. C.; Hiki, Y.; Ishihara, M.; Ito, H.; Kakehi, K.; Karlsson, N.; Kato, K.; Kawasaki, N.; Khoo, K. H.; Kobayashi, K.; Kolarich, D.; Kondo, A.; Lebrilla, C.; Nakano, M.; Narimatsu, H.; Novak, J.; Novotny, M. V.; Ohno, E.; Packer, N. H.; Renfrow, M. B.; Tajiri, M.; Thomsson, K. A.; Yagi, H.; Yu, S. Y.; Taniguchi, N. Comparison of Methods for Profiling O-glycosylation: HUPO Human Disease Glycomics/Proteome Initiative Multi-Institutional Study of IgA1. Mol Cell Proteomics2010, 9, 719-727. Pubmed Link
• Staples, G. O.; Naimy, H.; Yin, H.; Kileen, K.; Kraiczek, K.; Costello, C. E.; Zaia, J. Improved hydrophilic interaction chromatography LC/MS of heparinoids using a chip with postcolumn makeup flow. Anal. Chem.2010, 82, 516-22. Pubmed Link
• Ren, R.; Hong, Z.; Gong, H.; Laporte, K.; Skinner, M.; Seldin, D. C.; Costello, C. E.; Connors, L. H.; Trinkaus-Randall, V. Role of glycosaminoglycan sulfation in the formation of immunoglobulin light chain amyloid oligomers and fibrils. J Biol Chem2010, 285, 37672-82. Pubmed Link
• Palaima, E.; Leymarie, N.; Stroud, D.; Mizanur, R. M.; Hodgkin, J.; Gravato-Nobre, M. J.; Costello, C. E.; Cipollo, J. F. The Caenorhabditis elegans bus-2 mutant reveals a new class of O-glycans affecting bacterial resistance. J Biol Chem2010, 285, 17662-72. Pubmed Link
• Li, X.; Lin, C.; Han, L.; Costello, C. E.; O’Connor, P. B. Charge remote fragmentation in electron capture and electron transfer dissociation. J Am Soc Mass Spectrom2010, 21, 646-56. Pubmed Link
• Ebbel, E. N.; Leymarie, N.; Schiavo, S.; Sharma, S.; Gevorkian, S.; Hersch, S.; Matson, W. R.; Costello, C. E. Identification of phenylbutyrate-generated metabolites in Huntington disease patients using parallel liquid chromatography/electrochemical array/mass spectrometry and off-line tandem mass spectrometry. Anal Biochem2010, 399, 152-61. Pubmed Link
• Chatterjee, A.; Carpentieri, A.; Ratner, D. M.; Bullitt, E.; Costello, C. E.; Robbins, P. W.; Samuelson, J. Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer. PLoS Pathog2010, 6, e1001059. Pubmed Link

• Carpentieri, A.; Ratner, D. M.; Ghosh, S. K.; Banerjee, S.; Bushkin, G. G.; Cui, J.; Lubrano, M.; Steffen, M.; Costello, C. E.; O’Keefe, B.; Robbins, P. W.; Samuelson, J. The anti-retroviral lectin cyanovirin-N targets well-known and novel targets on the surface of Entamoeba histolytica trophozoites. Eukaryot Cell2010, 9, 1661-1668. Pubmed Link
• Young, D. C.; Kasmar, A.; Moraski, G.; Cheng, T. Y.; Waltz, A. J.; Hu, J.; Xu, X.; Endres, G. W.; Uzieblo, A.; Zajonc, D.; Costello, C. E.; Miller, M. J.; Moody, D. B. Synthesis of dideoxymycobactin antigens presented by CD1A reveals T cell fine specificity for natural lipopeptide structures. J Biol Chem2009, 284, 25087-25096. Pubmed Link
• Ying, W.; Perlman, D. H.; Li, L.; Theberge, R.; Costello, C. E.; McComb, M. E. Highly efficient and selective enrichment of peptide subsets combining fluorous chemistry with reversed-phase chromatography. Rapid Commun Mass Spectrom2009, 23, 4019-30. Pubmed Link
• Wang, Y.; Meriin, A. B.; Zaarur, N.; Romanova, N. V.; Chernoff, Y. O.; Costello, C. E.; Sherman, M. Y. Abnormal proteins can form aggresome in yeast: aggresome-targeting signals and components of the machinery. FASEB J2009, 23, 451-63. Pubmed Link
• Staples, G.; Bowman, M.; Costello, C. E.; Hitchcock, A.; Lau, J.; Leymarie, N.; Miller, C.; Naimy, H.; Shi, X.; Zaia, J. A Chip-based Amide-HILIC LC/MS Platform for Glycosaminoglycan Glycomics. Proteomics2009, 9, 686-695. Pubmed Link
• Singh, B. N.; Hayes, G. R.; Lucas, J. J.; Sommer, U.; Viseux, N.; Mirgorodskaya, E.; Trifonova, R. T.; Sassi, R. R.; Costello, C. E.; Fichorova, R. N. Structural details and composition of Trichomonas vaginalis lipophosphoglycan in relevance to the epithelial immune function. Glycoconj J2009, 26, 3-17. Pubmed Link
• Romesser, P. B.; Perlman, D. H.; Faller, D. V.; Costello, C. E.; McComb, M. E.; Denis, G. V. Development of a malignancy-associated proteomic signature for diffuse large B-cell lymphoma. The American journal of pathology2009, 175, 25-35. Pubmed Link
• Rex, S.; Beaulieu, L. M.; Perlman, D. H.; Vitseva, O.; Blair, P. S.; McComb, M. E.; Costello, C. E.; Freedman, J. E. Immune versus thrombotic stimulation of platelets differentially regulates signalling pathways, intracellular protein-protein interactions, and alpha-granule release. Thromb Haemost2009, 102, 97-110. Pubmed Link
• Perlman, D. H.; Bauer, S. M.; Ashrafian, H.; Bryan, N. S.; Garcia-Saura, M. F.; Lim, C. C.; Fernandez, B. O.; Infusini, G.; McComb, M. E.; Costello, C. E.; Feelisch, M. Mechanistic insights into nitrite-induced cardioprotection using an integrated metabolomic/proteomic approach. Circ Res2009, 104, 796-804. Pubmed Link
• Makkinje, A.; Near, R. I.; Infusini, G.; Vanden Borre, P.; Bloom, A.; Cai, D.; Costello, C. E.; Lerner, A. AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern. Cell Signal2009, 21, 1423-35. Pubmed Link