|The major focus of our work is to define mechanisms of innate immunity in the lungs. Bacteria in the lungs induce innate immune responses including neutrophil recruitment and plasma extravasation, which are mediated by the coordinated expression of diverse genes including adhesion molecules, chemokines, colony-stimulating factors, and cytokines. This gene expression program is controlled by transcription factors and post-transcriptional regulators such as microRNA. To illuminate these pathways, we use diverse laboratory approaches from the fields of biochemistry, molecular biology, cell biology, and physiology. We also develop new approaches as needed to better understand and manipulate innate immunity in the lungs.||
Epifluoresecent view of a mouse lung transfected with a gene for enhanced green fluorescent protein using transthoracic electroporation. Transfected cells can be visualized as glowing green through the pleural surface. Untransfected lungs or lungs transfected with other genes show no green fluorescence.