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Sequencing Library Preparation
Illumina NextSeq Sequencing
Affymetrix GeneChip
People
Library QC via bioanalyzer is performed as part of sequencing. Complete
ONLY
this form if you would like the BUMSR Core to sequence your SINGLE CELL libraries.
DO NOT
complete the Bioanalyzer QC form in addition
Hidden
Date
MM slash DD slash YYYY
Contact
Submitter Name
(Required)
First
Last
Submitter Email
(Required)
Enter Email
Confirm Email
Submitter Telephone No.
(Required)
P.I. Name
(Required)
First
Last
P.I. Email
(Required)
Enter Email
Confirm Email
Department
(Required)
Affiliation
(Required)
Boston University
External Organlization
Address
Street Address
Address Line 2
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Armed Forces Americas
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ZIP Code
Accounting
Do you have a BU Grant Number?
(Required)
Yes
No
Please email the MSR Core at MSRLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.
Do you have an Invoice Number?
(Required)
Unsure
Yes
No
Please email the MSR Core at MSRLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.
Grant Administrator Name
First
Last
Grant Administrator Email
Enter Email
Confirm Email
Fax Number
(Required)
Sequencing Libraries
Sequencing Library Species
(Required)
Human
Mouse
Other
Sequencing Library Type
(Required)
10X Single Cell/ Nuclei
CellSeq 2
Parse
Scale
InDrops
Other
If you choose "Other," please consult the Core BEFORE submitting your samples.
10X Library Type
(Required)
3' or 5' Gene Expression
On Chip Multiplexed (OCM) 3' or 5' Gene Expression
Flex Gene Expression
V(D)J: T-cell Enrichment
V(D)J: B-cell Enrichment
Multiome Gene Expression
Multiome ATAC-Seq
ATAC-Seq (not multiome)
CITE-Seq/ Antibody Tagged
Hashing/ Hash Tagged
Visium Spatial - FFPE
Visium Spatial - Fresh Frozen
Number of Individual Sequencing Libraries
(Required)
Did the BU Single Cell Core prepare your libraries?
(Required)
No
Yes
Was your library prepared using a custom protocol?
(Required)
A "custom protocol" refers to library generation that
DOES NOT use a commercially available kit or uses one with custom primers or other modifications. If a custom or modified protocol was used, you MUST consult the Core.
No
Yes
Other
What library prepration kit was used to generate your libraries?
Please indicate full title, manufacturer, and part number if applicable.
Do you need the MSR Core to pool your libraries?
(Required)
Individual library volumes must be "pooled" into a combined volume before injection into the sequencing flow cell.
No
Yes
Other
Library Name and Index Information
(Required)
You can download and fill out the library information excel sheet
here
before uploading it below. Please give the entire sequence of your indices, not only the well location or index name. Also, please indicate the volume of each library if you are submitting them individually.
Max. file size: 100 MB.
Illumina Sequencing
Sequencing Output
(Required)
NOTE:
The MSR Core has switched to Illumina's
NEW
XLEAP chemistry. If you would like your libraries sequenced using Old v3 chemistry, please indicate that in the
Final Notes
field at the end of this form.
NextSeq 2000 - P1 - 100M Reads
NextSeq 2000 - P2 - 400M Reads
NextSeq 2000 - P3 - 1.1B Reads
NextSeq 2000 - P4 - 1.7B Reads
NextSeq 500 - Mid - 100M Reads
NextSeq500 - High - 400M Reads
NextSeq 2000 P1 Read Length
(Required)
P1-100: 100 bp - Single Read
P1-100: 50 bp - Paired End Reads
P1-300: 300 bp - Single Read
P1-300: 150 bp - Paired End Reads
P1-600: 300 bp - Paired End Reads
Other
NextSeq 2000 P2 Read Length
(Required)
P2-100: 100bp - Single Reads
P2-100: 50bp - Paired End Reads
P2-200: 200 bp - Single Read
P2-200: 100 bp - Paired End Reads
P2-300: 300 bp - Single Read
P2-300: 150 bp - Paired End Reads
P2-600: 300 bp - Paired End Reads
Other
NextSeq 2000 P3 Read Length
(Required)
P3-50: 50 bp - Single Reads
P3-100: 100bp - Single Reads
P3-100: 50bp - Paired End Reads
P3-200: 200 bp - Single Read
P3-200: 100 bp - Paired End Reads
P3-300: 300 bp - Single Read
P3-300: 150 bp - Paired End Reads
Other
NextSeq 2000 P4 Read Length
(Required)
P4-50: 50 bp - Single Reads
P4-100: 100bp - Single Reads
P4-100: 50bp - Paired End Reads
P4-200: 200 bp - Single Read
P4-200: 100 bp - Paired End Reads
P4-300: 300 bp - Single Read
P4-300: 150 bp - Paired End Reads
Other
NextSeq 500 Mid Read Length
(Required)
Mid-150: 150 bp - Single Reads
Mid-150: 75bp - Paired End Reads
Mid-300: 300 bp - Single Read
Mid-300: 150 bp - Paired End Reads
Other
NextSeq 500 High Read Length
(Required)
High-75: 75bp - Single Reads
High-150: 150 bp - Single Reads
High-150: 75bp - Paired End Reads
High-300: 300 bp - Single Read
High-300: 150 bp - Paired End Reads
Other
Number of Sequencing Runs
(Required)
Analysis
The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account at
here
and provide the email used to register below.
What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis?
This can be an analyst assigned to the project or another investigator
Final Notes
Please include below any relevant details about your sequencing experiment that you reckon were not adequately captured in this form.
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