Next Generation Sequencing Service Request Form » Microarray and Sequencing Resource

Library QC via bioanalyzer is performed as part of sequencing. Complete ONLY this form if you would like the BUMSR Core to sequence your libraries. DO NOT complete the Bioanalyzer QC form in addition

Submitter Name*

First Last

Submitter Email*

Affiliation*

Telephone Number*

Fax Number*

Department*

Address

Street Address Address Line 2 City State / Province / Region ZIP / Postal Code Country

P.I. Name*

First Last

P. I. Email*

Do you have a BU Grant Account Number?*

If you do not have an grant number yet, please email the MSR Core at MSRLab@bu.edu as soon as you receive one. The service will not be provided until the Core receives the account number.

Grant Account Number*

Invoice Number*

If you are an non-BU investigator, please indicate your invoice number or "Invoice Pending."

Grant Administrator Name

First Last

Grant Administrator Email

Illumina NextSeq 500 & 2000 Sequencing

Sequencing Library Species*

Human
Mouse
Other

"Other" Species*

Type of Library to be Sequenced*

Single Cell Library Type*

Did you prepare your library using a custom protocol?*

No
Yes - Please email your protocol to the MSR Core

Does your library require custom sequencing primers for Read 1, Read 2, or Index Reads?*

This does not refer to multiplex indices. If you prepared your libraries using a custom protocol not tailored to sequencing on Illumina instruments, you may need a custom primer to sequence your library insert. Please refer to your protocol.

NextSeq Sequencing Instrument*

For more information about differences in read output, cycling, and cost differences between the two NextSeq instruments, please see the "Services" and "Pricing" sections of this site.

NextSeq 500
NextSeq 2000

NextSeq 500 Sequencing Output Type*

Mid Output - 130M Reads
High Output - 400M Reads

NextSeq 500 Sequencing Kit Type*

NextSeq 500 Sequencing Read Length*

150 bp single-end reads
75x75 bp paired-end reads
Custom read length

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Kit Type*

NextSeq 500 Sequencing Read Length*

75 bp single-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Read Length*

150 bp single-end reads
75x75 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 Sequencing Output Type*

P1 - 100M Reads
P2 - Over 400M Reads
P3 - 1B Reads

NextSeq 2000 P1 Sequencing Kit Type*

NextSeq 2000 P1 Sequencing Read Length*

100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths

Custom Read Length - P1 100 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P1 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length - P1 300 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P1 Sequencing Read Length*

600 bp single-end reads
300x300 bp paired-end reads
Custom read lengths

Custom Read Length - P1 600 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P2 Sequencing Kit Type*

NextSeq 2000 P2 Sequencing Read Length*

100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths

Custom Read Length - P2 100 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P2 Sequencing Read Length*

200 bp single-end reads
100x100 bp paired-end reads
Custom read lengths

Custom Read Length - P2 200 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P2 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length - P2 300 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P2 Sequencing Read Length*

600 bp single-end reads
300x300 bp paired-end reads
Custom read lengths

Custom Read Length - P2 600 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 Sequencing Kit Type*

NextSeq 2000 P3 Sequencing Read Length*

50 bp single-end reads
Custom read lengths

Custom Read Length - P3 50 kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P3 Sequencing Read Length*

100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths

Custom Read Length - P3 100 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P3 Sequencing Read Length*

200 bp single-end reads
100x100 bp paired-end reads
Custom read lengths

Custom Read Length - P3 200 Kit*

Please provide more information about your custom read length parameters

NextSeq 2000 P3 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length - P3 300 kit*

Please provide more information about your custom read length parameters

Number of Individual Libraries for NextSeq 500 Sequencing*

Number of Individual Libraries for NextSeq 2000 Sequencing*

Do you need the MSR Core to pool your libraries?*

Individual libraries must be pooled for sequencing. The MSR Core provides this as a service for an additional cost per sample.

No
Yes

Number of NextSeq Runs*

Library Submission Information*

Please download and fill out the library information excel sheet here. Please give the entire sequence of your indices, not only the well location or product part number. Also, please indicate the volume of each library if you are submitting them individually.

Do you require analysis from the BUMSR Computational Biology team?*

Basic RNA-Seq analysis is available at additional cost to the sequencing run.

No
Yes

The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account and provide the email used to register below.

What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis. (This can be an analyst assigned to the project or another investigator)

Final Notes

Please include any additional notes below.

Email

This field is for validation purposes and should be left unchanged.

BUMSR Core Sequencing Service Request

Library QC via bioanalyzer is performed as part of sequencing. Complete ONLY this form if you would like the BUMSR Core to sequence your libraries. DO NOT complete the Bioanalyzer QC form in addition

Illumina NextSeq 500 & 2000 Sequencing

The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account and provide the email used to register below.