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BUMSR Core Library Preparation + Sequencing Service Request
Sample QC via nanodrop and bioanalyzer is performed as part of library preparation. Complete
ONLY
this form if you would like the BUMSR Core to prepare libraries for your samples followed by sequencing.
DO NOT
complete the Bioanalyzer QC form or the Sequencing form in addition
Contact
Submitter Name
(Required)
First
Last
Submitter Email
(Required)
Submitter Telephone Number
(Required)
P.I. Name
(Required)
First
Last
P. I. Email
(Required)
Department
(Required)
Affiliation
(Required)
Boston University
External Organization
Address
Street Address
Address Line 2
City
Alabama
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District of Columbia
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Armed Forces Americas
Armed Forces Europe
Armed Forces Pacific
State
ZIP Code
Accounting
Do you have a BU Account Number?
(Required)
No
Yes
Please email the MSR Core at MSRLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.
Grant Administrator Name
(Required)
First
Last
Grant Administrator Email
(Required)
Do you have an Invoice Number?
(Required)
Unsure
Yes
No
Please email the MSR Core at MSRLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.
Fax Number
(Required)
Sequencing Library Preparation
Sample Species
(Required)
Human
Mouse
Other
Number of Samples to Submit
(Required)
How did you isolate your RNA samples?
(Required)
Please indicate the exact name of the RNA Isolation Kit you used to prepare your samples. If you did not use a kit, please specify your process and place additional details in the notes of this form.
Did you perform DNAse (or similar process) digestion during RNA isolation to remove genomic DNA?
(Required)
No
Yes
Library Preparation Type
(Required)
NEBNext mRNA with Poly-A Selection
Illumina mRNA with Poly-A Selection
NEBNext Total RNA with rRNA Reduction
Illumina Total RNA with rRNA Reduction
EpiCypher CUT & RUN
Other
If you're uncertain about which library preparation type to choose, or you select "Other" library type, please consult us.
Number of Libraries To Be Prepared
(Required)
Sample Submission Information
(Required)
You can download and fill out the sample information excel sheet
here
before uploading it below. Please limit your sample names to
at most
5 characters. Be sure to include
at minimum
the sample names and volumes, even if you have yet to determine the concentrations.
Max. file size: 100 MB.
Illumina Sequencing
Sequencing Output
(Required)
NOTE:
The MSR Core has switched to Illumina's
NEW
XLEAP chemistry. If you would like your libraries sequenced using Old v3 chemistry, please indicate that in the
Final Notes
field at the end of this form.
NextSeq2000 - P1 - 100M Reads
NextSeq 2000 - P2 - 400M Reads
NextSeq 2000 - P3 - 1.1B Reads
NextSeq 2000 - P4 - 1.7B Reads
NextSeq 500 - Mid - 100M Reads
NextSeq 500 - High - 400M Reads
NextSeq 2000 P1 Read Length
(Required)
P1-100: 100 bp - Single Read
P1-100: 50 bp - Paired End Reads
P1-300: 300 bp - Single Read
P1-300: 150 bp - Paired End Reads
P1-600: 300 bp - Paired End Reads
Other
NextSeq 2000 P2 Read Length
(Required)
P2-100: 100bp - Single Reads
P2-100: 50bp - Paired End Reads
P2-200: 200 bp - Single Read
P2-200: 100 bp - Paired End Reads
P2-300: 300 bp - Single Read
P2-300: 150 bp - Paired End Reads
P2-600: 300 bp - Paired End Reads
Other
NextSeq 2000 P3 Read Length
(Required)
P3-50: 50 bp - Single Reads
P3-100: 100bp - Single Reads
P3-100: 50bp - Paired End Reads
P3-200: 200 bp - Single Read
P3-200: 100 bp - Paired End Reads
P3-300: 300 bp - Single Read
P3-300: 150 bp - Paired End Reads
Other
NextSeq 2000 P4 Read Length
(Required)
P4-50: 50 bp - Single Reads
P4-100: 100bp - Single Reads
P4-100: 50bp - Paired End Reads
P4-200: 200 bp - Single Read
P4-200: 100 bp - Paired End Reads
P4-300: 300 bp - Single Read
P4-300: 150 bp - Paired End Reads
Other
NextSeq 500 Mid Read Length
(Required)
Mid-150: 150 bp - Single Reads
Mid-150: 75bp - Paired End Reads
Mid-300: 300 bp - Single Read
Mid-300: 150 bp - Paired End Reads
Other
NextSeq 500 High Read Length
(Required)
High-75: 75bp - Single Reads
High-150: 150 bp - Single Reads
High-150: 75bp - Paired End Reads
High-300: 300 bp - Single Read
High-300: 150 bp - Paired End Reads
Other
Analysis
Do you require analysis from the BUMSR Computational Biology team?
(Required)
Basic RNA-Seq analysis is available at additional cost to the sequencing run.
No
Yes
Other
The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account at
here
and provide the email used to register below.
What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis?
This can be an analyst assigned to the project or another investigator
Final Notes
Please include any additional notes below.
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