Library Preparation + Sequencing Request Form » Microarray and Sequencing Resource

Sample QC via nanodrop and bioanalyzer is performed as part of library preparation. Complete ONLY this form if you would like the BUMSR Core to prepare libraries for your samples followed by sequencing. DO NOT complete the Bioanalyzer QC form or the Sequencing form in addition

Submitter Name*

First Last

Submitter Email*

Affiliation*

Telephone Number*

Fax Number*

Department*

Address

Street Address Address Line 2 City State / Province / Region ZIP / Postal Code Country

P.I. Name*

First Last

P. I. Email*

Do you have a BU Grant Account Number?*

If you do not have an grant number yet, please email the MSR Core at MSRLab@bu.edu as soon as you receive one. The service will not be provided until the Core receives the account number.

Grant Account Number*

Invoice Number*

If you are an non-BU investigator, please indicate your invoice number or "Invoice Pending."

Grant Administrator Name

First Last

Grant Administrator Email

Sequencing Library Preparation

Species of Samples To Be Submitted*

Human
Mouse
Other

"Other" Species*

Number of Samples*

Number of Libraries To Be Prepared*

Type of Library Preparation*

How did you isolate your RNA samples?*

Please indicate the exact name of the RNA Isolation Kit you used to prepare your samples. If you did not use a kit, please specify your process and place additional details in the notes of this form.

Did you perform DNAse digestion during RNA isolation to remove genomic DNA?*

No
Yes

Sample Submission Information

If you have an excel/ word/ text file with information on the names, volumes, and concentrations of your samples, please attach the file here. Be sure to include at minimum the sample names and volumes, even if you have yet to determine the concentrations.

Name & Concentration (ng/uL) of Samples

Please list at minimum the sample names and volumes here. If you do not know the concentration of your samples, please indicate "TBD."

Name	Volume	Concentration

Illumina NextSeq 500 & 2000 Sequencing

NextSeq Sequencing Instrument*

For more information about differences in read output, cycling, and cost differences between the two NextSeq instruments, please see the "Services" and "Pricing" sections of this site.

NextSeq 500
NextSeq 2000

NextSeq 500 Sequencing Output Type*

Mid Output - 130M Reads
High Output - 400M Reads

NextSeq 500 Sequencing Kit Type*

NextSeq 500 Sequencing Read Length*

150 bp single-end reads
75x75 bp paired-end reads
Custom read length

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Kit Type*

NextSeq 500 Sequencing Read Length*

75 bp single-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Read Length*

150 bp single-end reads
75x75 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 500 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 Sequencing Output Type*

P2 - Over 400M Reads
P3 - 1B Reads

NextSeq 2000 Sequencing Kit Type*

NextSeq 2000 P2 Sequencing Read Length*

100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 P2 Sequencing Read Length*

200 bp single-end reads
100x100 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 P2 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 Sequencing Kit Type*

NextSeq 2000 P3 Sequencing Read Length*

50 bp single-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 P3 Sequencing Read Length*

100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 P3 Sequencing Read Length*

200 bp single-end reads
100x100 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

NextSeq 2000 P3 Sequencing Read Length*

300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths

Custom Read Length*

Please provide more information about your custom read length parameters

Number of NextSeq Runs*

Please enter the same number as "Library Pools for Sequencing" if you do not intend to sequence each pool more than once.

Do you require analysis from the BUMSR Computational Biology team?*

Basic RNA-Seq analysis is available at additional cost to the sequencing run.

No
Yes

The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account and provide the email used to register below.

What is the email associated with the BaseSpace account designated to receive raw sequencing data? (This can be an analyst assigned to the project or another investigator)

Final Notes

Please include any additional notes below.

Comments

This field is for validation purposes and should be left unchanged.

BUMSR Core Library Preparation + Sequencing Service Request

Sample QC via nanodrop and bioanalyzer is performed as part of library preparation. Complete ONLY this form if you would like the BUMSR Core to prepare libraries for your samples followed by sequencing. DO NOT complete the Bioanalyzer QC form or the Sequencing form in addition

Sequencing Library Preparation

Illumina NextSeq 500 & 2000 Sequencing

The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account and provide the email used to register below.