Proteoglycan and Glycosaminoglycan Structure

This page describes research led by Joseph Zaia, member of the Department of Biochemistry and Associate Director of the Center for Biomedical Mass Spectrometry at Boston University School of Medicine.

The mission of this research effort is to catalyze progress in proteoglycan biochemistry by gaining insight into the biological implications of the structural diversity of these molecules. Proteoglycans are found on the surfaces of every mammalian cell and in extracellular matrices, yet their functions are not well understood. Research focuses on sequencing glycosaminoglycans using mass spectrometry and determining the domain structure and protein binding properties of these molecules.

Research focuses on the functional implications of glycosaminoglycan structure and structural diversity. Understanding of the molecular basis for glycosaminoglycan function entials the ability to rapidly correlate structure and protein binding activity. Mass spectrometry shows great potential for meeting these needs.

The following is a summary of projects on glycosaminoglycan analysis and biochemistry

  1. Mass spectrometric glycomics profiling
  2. Software for analysis of glycomics LC/MS profiling data
  3. Tandem mass spectrometry of glycosaminoglycans
  4. Phenotypic analysis of tissue-specific glycosaminoglycans
  5. Remodeling of heparan sulfate by cell surface enzymes
  6. Glycosaminoglycan-protein binding
Primary teaching affiliate
of BU School of Medicine