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BUMSR Core Library Preparation + Sequencing Service Request
Sample QC via nanodrop and bioanalyzer is performed as part of library preparation. Complete
ONLY
this form if you would like the BUMSR Core to prepare libraries for your samples followed by sequencing.
DO NOT
complete the Bioanalyzer QC form or the Sequencing form in addition
Submitter Name
*
First
Last
Submitter Email
*
Affiliation
*
Boston University
External
Telephone Number
*
Fax Number
*
Department
*
Address
Street Address
Address Line 2
City
State / Province / Region
ZIP / Postal Code
Afghanistan
Albania
Algeria
American Samoa
Andorra
Angola
Anguilla
Antarctica
Antigua and Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belarus
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bonaire, Sint Eustatius and Saba
Bosnia and Herzegovina
Botswana
Bouvet Island
Brazil
British Indian Ocean Territory
Brunei Darussalam
Bulgaria
Burkina Faso
Burundi
Cabo Verde
Cambodia
Cameroon
Canada
Cayman Islands
Central African Republic
Chad
Chile
China
Christmas Island
Cocos Islands
Colombia
Comoros
Congo
Congo, Democratic Republic of the
Cook Islands
Costa Rica
Croatia
Cuba
Curaçao
Cyprus
Czechia
Côte d'Ivoire
Denmark
Djibouti
Dominica
Dominican Republic
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Eswatini
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
France
French Guiana
French Polynesia
French Southern Territories
Gabon
Gambia
Georgia
Germany
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guam
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Heard Island and McDonald Islands
Holy See
Honduras
Hong Kong
Hungary
Iceland
India
Indonesia
Iran
Iraq
Ireland
Isle of Man
Israel
Italy
Jamaica
Japan
Jersey
Jordan
Kazakhstan
Kenya
Kiribati
Korea, Democratic People's Republic of
Korea, Republic of
Kuwait
Kyrgyzstan
Lao People's Democratic Republic
Latvia
Lebanon
Lesotho
Liberia
Libya
Liechtenstein
Lithuania
Luxembourg
Macao
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar
Namibia
Nauru
Nepal
Netherlands
New Caledonia
New Zealand
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
North Macedonia
Northern Mariana Islands
Norway
Oman
Pakistan
Palau
Palestine, State of
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn
Poland
Portugal
Puerto Rico
Qatar
Romania
Russian Federation
Rwanda
Réunion
Saint Barthélemy
Saint Helena, Ascension and Tristan da Cunha
Saint Kitts and Nevis
Saint Lucia
Saint Martin
Saint Pierre and Miquelon
Saint Vincent and the Grenadines
Samoa
San Marino
Sao Tome and Principe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Singapore
Sint Maarten
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
South Georgia and the South Sandwich Islands
South Sudan
Spain
Sri Lanka
Sudan
Suriname
Svalbard and Jan Mayen
Sweden
Switzerland
Syria Arab Republic
Taiwan
Tajikistan
Tanzania, the United Republic of
Thailand
Timor-Leste
Togo
Tokelau
Tonga
Trinidad and Tobago
Tunisia
Turkmenistan
Turks and Caicos Islands
Tuvalu
Türkiye
US Minor Outlying Islands
Uganda
Ukraine
United Arab Emirates
United Kingdom
United States
Uruguay
Uzbekistan
Vanuatu
Venezuela
Viet Nam
Virgin Islands, British
Virgin Islands, U.S.
Wallis and Futuna
Western Sahara
Yemen
Zambia
Zimbabwe
Åland Islands
Country
P.I. Name
*
First
Last
P. I. Email
*
Do you have a BU Grant Account Number?
*
Yes
No
Pending
If you do not have an grant number yet, please email the MSR Core at MSRLab@bu.edu as soon as you receive one. The service will not be provided until the Core receives the account number.
Grant Account Number
*
Invoice Number
*
If you are an non-BU investigator, please indicate your invoice number or "Invoice Pending."
Grant Administrator Name
First
Last
Grant Administrator Email
Sequencing Library Preparation
Species of Samples To Be Submitted
*
Human
Mouse
Other
"Other" Species
*
Number of Samples
*
Number of Libraries To Be Prepared
*
Type of Library Preparation
*
mRNA with Poly-A Selection
Total RNA with Ribosomal Reduction
Small Whole Genome DNA
Other - Please Consult Us BEFORE Submitting
How did you isolate your RNA samples?
*
Please indicate the exact name of the RNA Isolation Kit you used to prepare your samples. If you did not use a kit, please specify your process and place additional details in the notes of this form.
Did you perform DNAse digestion during RNA isolation to remove genomic DNA?
*
No
Yes
Sample Submission Information
If you have an excel/ word/ text file with information on the names, volumes, and concentrations of your samples, please attach the file here. Be sure to include
at minimum
the sample names and volumes, even if you have yet to determine the concentrations.
Max. file size: 100 MB.
Name & Concentration (ng/uL) of Samples
Please list
at minimum
the sample names and volumes here. If you do not know the concentration of your samples, please indicate "TBD."
Name
Volume
Concentration
Illumina NextSeq 500 & 2000 Sequencing
NextSeq Sequencing Instrument
*
For more information about differences in read output, cycling, and cost differences between the two NextSeq instruments, please see the "Services" and "Pricing" sections of this site.
NextSeq 500
NextSeq 2000
NextSeq 500 Sequencing Output Type
*
Mid Output - 130M Reads
High Output - 400M Reads
NextSeq 500 Sequencing Kit Type
*
150 Cycles
300 Cycles
NextSeq 500 Sequencing Read Length
*
150 bp single-end reads
75x75 bp paired-end reads
Custom read length
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 500 Sequencing Read Length
*
300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 500 Sequencing Kit Type
*
75 Cycles
150 Cycles
300 Cycles
NextSeq 500 Sequencing Read Length
*
75 bp single-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 500 Sequencing Read Length
*
150 bp single-end reads
75x75 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 500 Sequencing Read Length
*
300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 Sequencing Output Type
*
P2 - Over 400M Reads
P3 - 1B Reads
NextSeq 2000 Sequencing Kit Type
*
100 Cycles
200 Cycles
300 Cycles
NextSeq 2000 P2 Sequencing Read Length
*
100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 P2 Sequencing Read Length
*
200 bp single-end reads
100x100 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 P2 Sequencing Read Length
*
300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 Sequencing Kit Type
*
50 Cycles
100 Cycles
200 Cycles
300 Cycles
NextSeq 2000 P3 Sequencing Read Length
*
50 bp single-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 P3 Sequencing Read Length
*
100 bp single-end reads
50x50 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 P3 Sequencing Read Length
*
200 bp single-end reads
100x100 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
NextSeq 2000 P3 Sequencing Read Length
*
300 bp single-end reads
150x150 bp paired-end reads
Custom read lengths
Custom Read Length
*
Please provide more information about your custom read length parameters
Number of NextSeq Runs
*
Please enter the same number as "Library Pools for Sequencing" if you do not intend to sequence each pool more than once.
Do you require analysis from the BUMSR Computational Biology team?
*
Basic RNA-Seq analysis is available at additional cost to the sequencing run.
No
Yes
The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account at
basespace.illumina.com
and provide the email used to register below.
What is the email associated with the BaseSpace account designated to receive raw sequencing data? (This can be an analyst assigned to the project or another investigator)
Final Notes
Please include any additional notes below.
Email
This field is for validation purposes and should be left unchanged.