Single Cell Service Request Form | Single Cell Sequencing Core

Submitter Name*

First Last

Submitter Phone Number*

Submitter Email*

Affiliation*

If you are not a BU student, staff, or faculty member, or if your project funding is not provided via a BU associated grant, please choose "External."

Department*

Institution/ Company*

P.I. Name*

First Last

P.I. Email*

Grant Administrator Name*

First Last

Grant Administrator Email*

Address

Street Address Address Line 2 City State / Province / Region ZIP / Postal Code Country

Do you have a BU Account Number?*

Please email the SCS Core at SCSLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.

Do you have an Invoice Number?*

Please email the SCS Core at SCSLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.

Services Requested

Project Name*

Please name the project to which your samples belong. If you are performing multiple submissions over time for a single project, please be sure to include the same project name for each submission.

Service Requested*

Protocol*

Universal 3' Expression
Universal 5' Expression
Epi ATAC
Epi Multiome ATAC & Expression
Flex Gene Expression

Universal 3'*

Gene expression
Feature Barcoding
On-Chip Multiplexing (OCM)

5' Profiling*

Gene expression
T Cell Receptor profiling
B Cell Receptor profiling
Feature Barcoding
On-Chip Multiplexing (OCM)

Flex Gene Expression Tissue/ Cell Type*

Fresh Fixed
Frozen Fixed
FFPE

Multiplexing for Flex Gene Expression*

Nuclei*

Please indicate if this is a single-nucleus project

Yes (sNuc-seq)
No

Targeted Cell Recovery*

Targeted number of captured cells

"Other" Target Cell Recovery*

Please enter a number from 100 to 10000.

Number of Samples*

Sample Species*

"Other" Sample Species

Sample Cell/ Tissue Type and Origin*

Please provide details and write out all acronyms (e.g. PBMC - peripheral blood mononuclear cell)

Sample Groups*

Please identify your sample groups (e.g: control and treatment groups) and which samples belong to each group

Project Overview*

A brief description of the project and study design

Do your samples contain a potentially infectious agent?*

For example: Have your sample cells or tissues undergone infection with bacteria or virus? All clinical samples from human subjects are considered potentially infectious.

Are your cells from clinical human subjects samples?*

Do you have an IBC-approved Protocol covering your work with the samples you are submitting to the Single Cell Core?*

Do your sample have high, average, or low transcriptional activity?*

Do you require sequencing data analysis from the SCS Core Computational Biology team?*

Basic level analysis is provided at an additional cost.

Marker genes

If you answered 'yes' to the previous question, are there any particular marker genes that are important to your study, or could be key to identifying cell populations?

Final Notes

Acknowledgements

Please agree below in order to submit.

Consultation*

I acknowledge that I have had a consultation meeting with the Single Cell Core regarding my project.

Agree

Safety Agreement*

I acknowledge that information I have provided in this form about sample source and biosafety is accurate.

Agree

I agree that I will comply with safe conduct requests made by Single Cell Core researchers during sample delivery and processing.

Agree

Appointment*

Please book a sample intake appointment in conjunction with submitting this form. We will not be able to take your samples without a confirmed appointment.

Agree

Email

This field is for validation purposes and should be left unchanged.