Services

As a high-throughput core, we provide an array of services to get you from sample to high-quality single cell gene expression data and/or analysis. We offer multiple single cell library preparation options and next-generation Illumina sequencing via our collaboration with the BU Sequencing Core. Uncover critical insight with our services.

Single Cell Services

10X Genomics Chromium X™

The 10X Genomics’ Chromium X™ platform uses microfluidics to partition single cells or nuclei and prepare sequencing libraries. 10X Genomics offers a suite of solutions to investigate targets from gene expression to immune and open chromatin profiling.

 

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10X Genomics Chromium X controller and Universal 3′ Gene Expression assay. Image retrieved from https://www.10xgenomics.com/products/universal-three-prime-gene-expression

 

The BU Single Cell Core offers 10X Genomics library preparation from single cell or single nuclei samples. You can find more information about the assays we offer on our 10X Genomics Single Cell Services page. Our Pricing page will provide more information on experiment costs. Investigators are always welcome to contact us with any questions.

Illumina NextSeq NGS Sequencing

We collaborate with the Boston University Sequencing Core to provide you with the best sequencing for your single cell/ nuclei libraries via Illumina NextSeq 2000 platform.

Data Analysis

We offer detailed analysis of your single cell data. For further details and pricing, please contact us.
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Tissue Dissociation Services

As part of our mission to provide high quality single cell sequencing data, we offer walk-up access to our Miltenyi Biotech GentleMACS Octo tissue dissociator with heaters. By appointment, researchers may utilize our GentleMACS instrument to develop fully automated protocols for dissociating their tissues of interest into cell suspensions ready for downstream applications.

Miltenyi Biotec GentleMACS Octo Tissue Dissociator

The Miltenyi Biotec GentleMACS™ Octo Dissociator with Heaters is a benchtop instrument for the fully automated and standardized tissue dissociation or homogenization of up to eight samples. 

GentleMACS Octo Tissue Dissociator with Heaters

Equipped with eight individual heating units and ready-to-use gentleMACS programs, it offers a fully-automated workflow for tissue dissociation. It also allows for the creation of user-defined programs.  All eight positions can be operated independently.

Single-cell suspensions or thorough homogenates are obtained using single-use, sterile C Tubes or M Tubes. C tubes and M tubes may be obtained through Miltenyi Biotech.