Spatial Sequencing Request Form | Microarray & Sequencing Resource

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Date

Contact

Submitter Name(Required)

First Last

Submitter Email(Required)

Submitter Telephone No.(Required)

P. I. Name(Required)

First Last

P. I. Email(Required)

Affiliation(Required)

Department(Required)

Address(Required)

Street Address Address Line 2 City State ZIP Code

Accounting

Do you have a BU Grant Account Number?(Required)

Please EMAIL the BUSR Core at MSRLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.

Do you have an Invoice Number?(Required)

Please EMAIL the BUSR Core at MSRLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.

Grant Administrator Name(Required)

First Last

Grant Administrator Email(Required)

Sequencing Libraries

Number of Individual Sequencing Libraries(Required)

The number of sequencing libraries typically matches the number of tissue samples in your spatial experiment.

Sequencing Library Species(Required)

Human

Mouse

Other

10X Visium Spatial Library Type(Required)

Visium CytAssist Spatial Gene Expression

Visium HD Spatial Gene Expression

Visium HD 3' Spatial Gene Expression

10X Visium Spatial Sample Type(Required)

Fresh-Frozen

Fixed-Frozen

FFPE

10X Visium Spatial Slide Type(Required)

6.5 mm

11.0 mm

Did the BU Spatial Biology Core (SBC) prepare your libraries?(Required)

Was your library prepared using a custom protocol?(Required)

A "custom protocol" refers to library generation that DOES NOT use a commercially available kit or uses one with custom primers or other modifications. If a custom or modified protocol was used, you MUST consult the BUSR core.

What library preparation kit was used to generate your libraries?

Please indicate full title, manufacturer, and part number if applicable.

Do you need the MSR Core to pool your libraries?(Required)

Individual library volumes must be "pooled" into a combined volume before injection into the sequencing flow cell.

Library Name and Index Information(Required)

Please download and fill out the library information excel sheet here before uploading it above. Please give the entire sequence of your indices, not only the well location or index name. Also, please indicate the volume of each library if you are submitting them individually.

Max. file size: 100 MB.

Illumina Sequencing

10X Genomics recommends to obtain a minimum number of sequencing reads for spatial biology sequencing based on library type, sample type, slide type, and percent of capture area covered by the sample tissue. Please refer to the 10X Spatial Sequencing Parameters for more information, and reach out to the BUSR Core at MSRLab@bu.edu if you have further questions.

Sequencing Output(Required)

NOTE: The BUSR Core uses Illumina's XLEAP chemistry. If you would like your libraries sequenced using Legacy v3 chemistry, please indicate that in the Final Notes field at the end of this form.

NextSeq 2000 - P1 - 100M Reads

NextSeq 2000 - P2 - 400M Reads

NextSeq 2000 - P3 - 1.1B Reads

NextSeq 2000 - P4 - 1.7B Reads

NextSeq 500 - Mid - 100M Reads

NextSeq 500 - High - 400M Reads

NextSeq 2000 P1 Read Length(Required)

P1-100 cycles: 100 bp - Single Read

P1-100 cycles: 50 x 50 bp - Paired End Reads

P1-300 cycles: 300 bp - Single Read

P1-300 cycles: 150 x 150 bp - Paired End Reads

P1-600 cycles: 300 x 300 bp - Paired End Reads

Other

NextSeq 2000 P2 Read Length(Required)

P2-100 cycles: 100bp - Single Reads

P2-100 cycles: 50 x 50 bp - Paired End Reads

P2-200 cycles: 200 bp - Single Read

P2-200 cycles: 100 x 100 bp - Paired End Reads

P2-300 cycles: 300 bp - Single Read

P2-300 cycles: 150 x 150 bp - Paired End Reads

P2-600 cycles: 300 x 300 bp - Paired End Reads

Other

NextSeq 2000 P3 Read Length(Required)

P3-50 cycles: 50 bp - Single Reads

P3-100 cycles: 100bp - Single Reads

P3-100 cycles: 50 x 50 bp - Paired End Reads

P3-200 cycles: 200 bp - Single Read

P3-200 cycles: 100 x 100 bp - Paired End Reads

P3-300 cycles: 300 bp - Single Read

P3-300 cycles: 150 x 150 bp - Paired End Reads

Other

NextSeq 2000 P4 Read Length(Required)

P4-50 cycles: 50 bp - Single Reads

P4-100 cycles: 100bp - Single Reads

P4-100 cycles: 50 x 50 bp - Paired End Reads

P4-200 cycles: 200 bp - Single Read

P4-200 cycles: 100 x 100 bp - Paired End Reads

P4-300 cycles: 300 bp - Single Read

P4-300 cycles: 150 x 150 bp - Paired End Reads

Other

Number of Sequencing Runs(Required)

Please indicate the number of sequencing runs you would like performed to achieve desired coverage.

Analysis

The BUSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously used BaseSpace, please sign up for a free BaseSpace account here. Data is transferred to recipients at their registered BaseSpace email, so please provide below the email used.

What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis?

This can be an analyst assigned to the project or another investigator

Final Notes

Please use the space below to provide any additional necessary details.