Single Cell Sequencing Request Form | Microarray & Sequencing Resource

Library QC via bioanalyzer is performed as part of sequencing. Complete ONLY this form if you would like the BUSR Core to sequence your SINGLE CELL sequencing libraries. DO NOT complete the Bioanalyzer QC form in addition.

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Date

Contact

Submitter Email(Required)

Submitter Telephone No.(Required)

P. I. Email(Required)

Affiliation(Required)

Department(Required)

Company or Institution(Required)

Accounting

Do you have a BU Grant Account Number?(Required)

Please email the BUSR Core at MSRLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.

Do you have an Invoice Number?(Required)

Please email the BUSR Core at MSRLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.

Grant Administrator Email(Required)

Sequencing Libraries

Number of Individual Sequencing Libraries(Required)

"Other" Sequencing Library Type(Required)

If you have chosen "Other," please consult the BUSR core BEFORE submitting your samples.

Did the BU Single Cell Core (SCS) prepare your libraries?(Required)

Was your library prepared using a custom protocol?(Required)

A "custom protocol" refers to library generation that DOES NOT use a commercially available kit or uses one with custom primers or other modifications. If a custom or modified protocol was used, you MUST consult the BUSR core.

What library prepration kit was used to generate your libraries?

Please indicate full title, manufacturer, and part number if applicable.

Do you need the MSR Core to pool your libraries?(Required)

Individual library volumes must be "pooled" into a combined volume before injection into the sequencing flow cell.

Library Name and Index Information(Required)

Please download and fill out the library information excel sheet here before uploading it above. Please give the entire sequence of your indices, not only the well location or index name. Also, please indicate the volume of each library if you are submitting them individually.

Max. file size: 100 MB.

Illumina Sequencing

Number of Sequencing Runs(Required)

Please indicate the number of sequencing runs you would like performed to achieve desired coverage.

Analysis

The BUSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously used BaseSpace, please sign up for a free BaseSpace account here. Data is transferred to recipients at their registered BaseSpace email, so please provide below the email used.

What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis?

This can be an analyst assigned to the project or another investigator

Final Notes