Sequencing Service Request Form | Microarray & Sequencing Resource

Library QC via bioanalyzer is performed as part of sequencing. Complete ONLY this form if you would like the BUMSR Core to sequence your BULK sequencing libraries. DO NOT complete the Bioanalyzer QC form in addition.

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Date

Contact

Submitter Name(Required)

First Last

Submitter Email(Required)

Submitter Telephone No.(Required)

P. I. Name(Required)

First Last

P. I. Email(Required)

Affiliation(Required)

Department(Required)

Address(Required)

Street Address Address Line 2 City State ZIP Code

Accounting

Do you have a BU Grant Account Number?(Required)

Please email the MSR Core at MSRLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.

Do you have an Invoice Number?(Required)

Please email the MSR Core at MSRLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.

Grant Administrator Name(Required)

First Last

Grant Administrator Email(Required)

Sequencing Libraries

Number of Individual Sequencing Libraries(Required)

Sequencing Library Species(Required)

Human

Mouse

Other

Sequencing Library Type(Required)

Total RNA with Ribosomal Reduction

mRNA with Poly-A Selection

Small RNA

Targeted RNA

Small Whole Genome

Targeted DNA

ChIP DNA

CUT & RUN DNA

Other

"Other" Sequencing Library Type(Required)

If you have chosen "Other," please consult the Core BEFORE submitting your samples.

Was your library prepared using a custom protocol?(Required)

A "custom protocol" refers to library generation that DOES NOT use a commercially available kit or uses one with custom primers or other modifications. If a custom or modified protocol was used, you MUST consult the Core.

What library prepration kit was used to generate your libraries?

Please indicate full title, manufacturer, and part number if applicable.

Do you need the MSR Core to pool your libraries?(Required)

Individual library volumes must be "pooled" into a combined volume before injection into the sequencing flow cell.

Library Name and Index Information(Required)

Please download and fill out the library information excel sheet here before uploading it above. Please give the entire sequence of your indices, not only the well location or index name. Also, please indicate the volume of each library if you are submitting them individually.

Max. file size: 100 MB.

Illumina Sequencing

Sequencing Output(Required)

NOTE: The MSR Core has switched to Illumina's NEW XLEAP chemistry. If you would like your libraries sequenced using Old v3 chemistry, please indicate that in the Final Notes field at the end of this form.

NextSeq 2000 - P1 - 100M Reads

NextSeq 2000 - P2 - 400M Reads

NextSeq 2000 - P3 - 1.1B Reads

NextSeq 2000 - P4 - 1.7B Reads

NextSeq 500 - Mid - 100M Reads

NextSeq 500 - High - 400M Reads

NextSeq 2000 P1 Read Length(Required)

P1-100 cycles: 100 bp - Single Read

P1-100 cycles: 50 x 50 bp - Paired End Reads

P1-300 cycles: 300 bp - Single Read

P1-300 cycles: 150 x 150 bp - Paired End Reads

P1-600 cycles: 300 x 300 bp - Paired End Reads

Other

NextSeq 2000 P2 Read Length(Required)

P2-100 cycles: 100bp - Single Reads

P2-100 cycles: 50 x 50 bp - Paired End Reads

P2-200 cycles: 200 bp - Single Read

P2-200 cycles: 100 x 100 bp - Paired End Reads

P2-300 cycles: 300 bp - Single Read

P2-300 cycles: 150 x 150 bp - Paired End Reads

P2-600 cycles: 300 x 300 bp - Paired End Reads

Other

NextSeq 2000 P3 Read Length(Required)

P3-50 cycles: 50 bp - Single Reads

P3-100 cycles: 100bp - Single Reads

P3-100 cycles: 50 x 50 bp - Paired End Reads

P3-200 cycles: 200 bp - Single Read

P3-200 cycles: 100 x 100 bp - Paired End Reads

P3-300 cycles: 300 bp - Single Read

P3-300 cycles: 150 x 150 bp - Paired End Reads

Other

NextSeq 2000 P4 Read Length(Required)

P4-50 cycles: 50 bp - Single Reads

P4-100 cycles: 100bp - Single Reads

P4-100 cycles: 50 x 50 bp - Paired End Reads

P4-200 cycles: 200 bp - Single Read

P4-200 cycles: 100 x 100 bp - Paired End Reads

P4-300 cycles: 300 bp - Single Read

P4-300 cycles: 150 x 150 bp - Paired End Reads

Other

NextSeq 500 Mid Read Length(Required)

Mid-150 cycles: 150 bp - Single Reads

Mid-150 cycles: 75 x 75 bp - Paired End Reads

Mid-300 cycles: 300 bp - Single Read

Mid-300 cycles: 150 x 150 bp - Paired End Reads

Other

NextSeq 500 High Read Length(Required)

High-75 cycles: 75bp - Single Reads

High-150 cycles: 150 bp - Single Reads

High-150 cycles: 75 x 75 bp - Paired End Reads

High-300 cycles: 300 bp - Single Read

High-300 cycles: 150 x 150 bp - Paired End Reads

Other

Number of Sequencing Runs(Required)

Please indicate the number of sequencing runs you would like performed to achieve desired coverage.

Analysis

Do you need analysis from the BUMSR Computational Biology team?(Required)

Basic RNA-Seq analysis is available at additional cost to the sequencing run.

The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account at here and provide the email used to register below.

What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis?

This can be an analyst assigned to the project or another investigator

Final Notes